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II. RESEARCH - PUBLICATIONS A.
JOURNAL ARTICLES (REFEREED, BIOCHEM. SOC.
TRANS. BY EDITOR) Stoppain, A.O.M., Bennun,
A., and dePahn, E.M. Energy requirement for the anaerobic oxidation of
acetate in baker's yeast. (1964). Biochim.
Biophys. Acta 92, 176-178. Bennun, A., dePahn, E.M., and Stoppani,
A.O.M. Sorne properties of particle-bound intracellular ATPase
(adenosinetriphosphatase) from baker's
yeast. (1964). Biochim. Biophys. Acta 89, 532-539. Stoppani, A.O.M., Bennun,
A., Effect of 2-4 Dinitrophenol on Krebs cycle and phosphate metabolism in
baker’s yeast. Arch. Biochem. Biophys.
108, 258-265. Bennun, Alfredo and Avron,
Mordhay. Light-dependent and light-triggered adenosinetriphosphatases in
chloroplasts. (1964). Biochim.
Biophys. Acta 19, 646-648. Bennun, Alfredo and Avron,
Mordhay (1965). The relation of the
light-dependent and light-triggered adenosinetriphosphatases to
photophos-phorylation. Biochim. Biophys. Acta 109, 117-127. Bennun, A. and Blum, J.J.
Properties of the induced acid phosphatase and of the constitutive acid
phosphatase of Euglena. (1966). Biochim.
Biophys. Acta 125, 106-123. Bennun, A. and Racker, E.
Partial resolution of the enzymes catalyzing photophosphorylation IV. Interaction of coupling factor I from
chloroplast with components of the chloroplast membrane. (1969). J. Biol. Chem. 244, 1325-1331. Bennun, A. Hypothesis for
coupling energy transduction with ATP synthesis or ATP hydrolysis (1971). Nature (New Biology) 233, 5-8. Bennun, A. The unitary hypothesis on the coupling of
energy transduction and its relevance to the modeling of mechanism. In "Mechanism of Energy Transduction in
Biological System" (D.E. Green, ed.). (1974). Brydon-Golz, S. and
Bennun, A. Postsynthetic stabilized modification of adenylate cyclase by
metabolites. (1975). Biochem. Soc.
Trans. 3, 721-724. Bennun, A. hypothesis on
the role of liganded states of proteins in energy transducing systems. (1975) Biosytems
7, 230-244. Harris, R. and Bennun,
A. Hormonal control of fat cells
adenylate cyclase (1976). Molecular and Cellular Biochemistry 13,
141-146. Brydon-Golz, S., Ohanian,
H. and Bennun, A. Effects of noradrenaline on the activation and the
stability of brain adenylate cyclase (1977). The Biochemical Journal 166, 473-483. Ohanina, H., Borhanian, K.
and Bennun, A. The effect of manganese
on the regulation of brain adenylate cyclase by magnesium and ATP. (1978) Biochem. Soc. Trans. 6, 1179-1182. Harris, R.H., Cruz, R. and
Bennun, A. The effect of hormones on
metal and metal-ATP interactions with fat cell adenylate cyclase. (1979). Biosystems 11, 29-46. Ohanian, H., Borhanian,
K., deFarias, S. and Bennun, A. A
model for the regulation of brain adenylate cyclase by ionic equilibria (1981). Special Issue of Journal of Bioenergetics and
Biomembranes Vol. 13, Nos. 5/6,
317-355. DeBari, V.A., and Bennun,
A. Cyclic GNP in the human erythrocyte.
Intracellular levels and transport in normal subjects and chronic
hemodialysis patients. (1982) Clinical Biochemistry 15, 219-221. DeBari, V.A., Novak, N.A.
and Bennun, A. Cyclic nucleotide
metabolism in the human erythrocyte (1984).
Clin. Physiol. Biochem. 2,
227-238. Novembre, P., Nicotra, J.,
DeBari, V.A., Needle, N.A., and Bennun, A. Erythrocyte transport of cyclic
nucleotides (1984). Annals of the Bennun, A., Needie, N.A.,
and DeBari, V.A Stimulation of the
hexose monophosphate pathway in the human
by Mn+2: Evidence
for a Mn – dependent NADPH peroxidase
activity. (1985). Biochemical
Medicine. Vol. 33, 17-21 DeBari, V.A., Needie,
N.A., and Bennun, A. Catalase as a manganese-dependent NADPH peroxidase.
(1985). Biochem. Soc. Trans. 13,
125-127. Bennun, A., Needie, N.A.
and DeBari, V.A. Infrared spectroscopy
of erythrocyte plasma membranes (1985). Biochem.
Soc. Trans. 13, 127-128. Sulner, J.W. and Bennun,
A. Ca 2+ - dependent
control of renin release (1985). Biochem. Soc. Trans. 13, 363-364. Bennun, A., Seidler, N.
and DeBari, V.A. A model for the
regulation of haemoglobin affinity for oxygen (1985). Biochem. Soc. Trans. 13, 364-366. Emma, J.E., Cervoni, P.,
Sulner, J.W. and Bennun, A. KCI-stimulated renin release (1986). Annals
of the Bennun, A., Seidler, N.
and DeBari, V.A. Divalent metals in
the regulation of hemoglobin affinity for oxygen (1986). Annals
of the Bennun, A. A coupling mechanism to interrelate
regulatory with haemohaem interactions of haemoglonin. (1987). Biomed.
Biochem. Acta. 46, No. 2/3, 314-319. Casciano, C. and Bennun,
A. A characterization of two inhibitors of H+, K+ - ATPase in gastric
tissue. (1988). Biochem. Soc. Trans. 16, 27-29. Vicario, P.P., Saperstein,
R. arid Bennun, A. Regulation of insulin receptor tyrosine kinase by divalent
metal cations, metal-ATP substrate and free ATP -4. (1988). Biochem. Soc. Trans. 16, 40-42. Vicario, P.P., Saperstein,
R., Katzen, H. and Bennun, A.
Metabolic interactions of insulin-activated receptor tyrosine
kinase. (1988). Casciano, C. and Bennun,
A. Characterization of the
interrelationship of the secretory activities of gastric tissue. (1988)
Vicario, P.P., Sapertein,
R. and Bennun, A. Role of divalent metals in the kinetic mechanism of insulin
receptor Tyrosine Kinase. (1988). Arch.
Biochem. Biophys. 261, 336-345. Vicario, P.P., Saperstein,
R. and Bennun, A. Role of Divalent Metals in the activation and regulation of
insulin receptor tyrosine kinase. (1988). BioSystems 22, 55-56. Vicario, P.P., Saperstein,
R. and Bennun, A. Interrelationships
of peptide substrate and metal-ATP with insulin receptor tyrosine kinase.
(1988). Biochem. Soc. Trans. 16. Vicario, P.P., Saperstein,
R. and Bennun, A. Interactions of
Insulin receptor tyrosine kinase with heavy metals. (1988). Biochem. Soc. Trans. 16, 896-897. Vicario, P.P., Saperstein,
R. and Bennun, A. Interaction of MnATP and peptide substrate with insulin
receptor tyrosine kinase. (1989). Biochem.
Soc. Trans. 17, 1108-1109. Vicario, P.P. and Bennun,
A. Regulation of insulin receptor tyrosine kinase by metabolic intermediates.
(1989). Biochem. Soc. Trans. 17,
1110-1111. Casciano, C. and Bennun,
A. The effect of Li + on the secretion
of HCO3- in Rat fundic tissue. (1989). Biochem. Soc. Trans. 17, 1111-1112. Casciano, C., Kaminska,
G.Z., and Bennun, A. Investigations on the Cytoprotective Mechanisms of
Thiocyanate in Rat Gastric Mucosa. (1989). Biochem. Soc. Trans 17, 1113-1114. Vicario, P.P. and Bennun,
A. Separate effects of Mg2+, MgATP, and ATP4- on the
kinetic mechanism for insulin receptor tyrosine kinase. (1990). Archives of Biochem. and Biophys. Vol.
278, 99-105. Sulner, J.W., Emma, J.E.
and Cervoni, P., Bennun, A. KCL stimulation of In Vitro renin release: Prostaglandin and Noradrenergic components. B.
CHAPTERS IN BOOKS, REVIEN ARTICLES AND PROCEEDINGS Stoppani, A.O.M., Ramos,
E.H., Widuczynski, Stoppani, A.O.M., Bennun,
A., and De Pahn, E.M. Effect of DNP on
the metabolism of phosphates in Saccharomyces cerevisiae (1965). Symp. of the inter-Am. Nuclear Energy
Commission, Bennun, A. Interaction of the chloroplast coupling
factor with protons and water (1971). Congreso Argentino de Ciencias Biologicas, Bennun, A. Properties of chloroplast's coupling
factor-1 and a hypothesis for a mechanism of energy transduction (1971).
Proceedings First European Biophysics Congress, Bennun, A. and Bennun,
N. Hypothesis for a mechanism of
energy of energy transduction.
Sigmoidal kinetics of chloroplast's heat-activated ATPase. (1972) In
Proc 2nd Int Cong. On Photosynthesis Res. (Giorgio Fortí, Mordhay Avron
and, Andrea Melardri, eds.) Vol. 2,
1115-1124, Dr. W. Junk N.V. Pub., Bennun, A. A model mechanism for coupled
phosphorylation. (1974) Proc. 3rd Int. Cong. Photosynthesis, Rehovoth (M.Avron, ed.)
Vol. 2 pp. 1107-1120 Elsevier Sci.
Pub. Co. C. PUBLICATIONS IN RAPID –
COMMUNICATION JOURNALS Bennun, A. Hypothesis relating an ion-stimulated
ATPase to the molecule code theory of memory. (1969). Bioenergetics Bulletin 1, No. 6, 49-50. Bennun, A. Effect of pH on heat-activated, Ca2+-dependent
ATPase. (1970). Bioenergetics Bulletin
2, No. 7, 63-65. Bennun, a. Hypothesis for a mechanism of energy
transduction. (1971). Bioenergetics
Bulletin 3, No. 2, 11-15. D.
COMMUNICATIONS AND ABSTRACTS Bennun, A. Phosphorus
metabolism and its relation to the activity of adenosínetríphosphatase in
Baker's yeast (Saccharomyces cerevisiae). (1965). Dissertation Abst. Vol. XXVI
(2), 665-6. Bennun, A. and Racker,
E. Interaction between chloroplast
particles and coupling factor. (1966). VII
Cong. A.L.A.C.F., 7-12 Aug. 1966, Mar del Plata, Arg., Acta Physiol. Latino Americana, Vol. XVI, Suppl. No. 1, 16. Bennun, A. and Racker,
E. Interaction between chloroplast
particles and coupling factor. (1967).
Federation Proc., Vol. 26-2
609. Bennun, a. and Blum,
J.J. Properties of the induced
phosphatase and of the constitutive acid phosphate of Euglena. (1966). B.B.A. 65466. Bennun, A. and Bennun, N.
Regulation of heat-activated ca2+-dependent ATPase, l5th Annual Meeting Biophysical Society
Abstracts, 39a, 1971. Bennun, A. The unitary hypothesis for coupling of
energy transduction and its relevance to the modeling of mechanisms for ATP synthesis
or ATP hydrolysis. Conference on the Mechanism of Energy Transduction in
Biological Systems Abst. The Bennun, A. Hypothesis for a biochemical memory pathway
IUB. (1973). Ninth International Congress
of Biochemistry, Abstract book Dc 27, 459, Bennun, A. The unitary hypothesis and the relationship
of enzyme modification to the pathophysiology of membranes. (1974). Biochemistry
/ Biophysics 1974 Meeting Abstracts, Fed. Proc. 33, 1528. Bennun, A. A model
mechanism for coupled phosphorylation. (1974). Abstracts book. 3rd
International Congress on Photosynthesis, Rehovoth. Harris, R.H. and Bennun,
A. Integration of hormone-dependent
effects of adenyl cyclase. (1974). Abstr.
Am. Chem. Soc. 168th Meeting Biol. 130 Bennun, A. and
Brydon-Golz, 5. Norepinephrine and the pathophysiology of neuronal
membranes. (1975). Biophysical Journal 15, No. 2, 49a. Bennun, A. A model pathway for coupled
phosphorylation. (1975). Plant
Physiology 56, No. 2, 47. Brydon-Golz, 5. and
Bennun, A. Post-synthetic stabilized modification of adenylate cyclase by
metabolites. (1975). The Biochemical
Society Agenda Papers, 557th Meeting, Bennun, A. A metal coordinative
chemistry model for coupled phosphorylation. (1975). Abstracts 3rd Annual Meeting American Society for Photobiology,
pp. 79. Brydon-Golz, S. and
Bennun, A. Relevance of the alteration of thresholds of adenylcyclase
response. (1975). Abstr. papers Am.
Chem. Soc. 17Oth Meeting, Biol. Chem., 165. Brydon-Golz, S. and Bennun, A. Regulation
of thresholds of adenylcyclase response. (1976). Biophysical Journal 16, No. 2 part 2, 190a. Bennun, A. A structure and
function model for coupling between regulatory and active site. (1977). Biophysical Journal 17, 239a. Harris, R., Cruz, R. and
Bennun, A. Metal and ATP interactions with adenylate cyclase. (1978). Biophysical Journal 21, 14a. Harris, R., Cruz, R. and
Bennun, A. Calcium and calcium-ATP
interactions with adenylate cyclase. (1978). Federation Proc. 37, 1789. Harris, R. and Bennun, A.
Hormonal control of fat cells adenylate cyclase. (1978). Feeding, Weight and Obesity Abstracts, 2w1055. Ohanian, H., Borhanian, K.
and Bennun, A. The effect of the equilibrium
concentrations of metal and ATP on adenylate cyclase. (1978). Abstr. Am. Chem. Soc. 176th National Meeting Biol. 51. Ohanian, H., Borhanian, K.
and Bennun, A. The effect of manganese
on the regulation of brain adenylate cyclase by magnesium and ATP. (1978) The
Biochemical Society Agenda Papers 57th meeting, Ohanian, H. and Bennun, A.
Regulation of the responsiveness to calcium of hypothalamic adenylate
cyclase. (1980). Fed. Proc. 39 (6) 2106. Harris, R.H., Cruz, R. A.
and Bennun, A. The effects of hormones
on metal and metal-ATP interaction
with fat cell adenylate cyclase. Eastern Regional Conference on
Comparative Endocrinology, Nicotra, Y., Needle, M.,
Ward, W., Bennun, A. and DeBari, V.
Transport of guanosine 3,,5' cyclic monophosphate cGMP into human
erythrocytes. (1983). Biophysical
Journal vol. 41, No. 2, Part 2, 163a. DeBari, V.A., Bennun, A.
and Needle, M.A. Multiple internal
reflectance-infrared spectroscopy (MIR-IR) of erythrocyte plasma membranes
(Ghosts). (1983). Federation Proc.
vol. 42, No, 7, 2552. DeBari, V., Bennun,
A. Regulation of the pentose phosphate
pathway in the human erythrocyte. (1984). Biophysical Journal, vol. 45, No. 2, Part 2, 212. Cruz, R.A., Menendez, S.
and Bennun, A. The Role of energy
charge in regulation of brain adenylate cyclase activity. (1984). Bull. DeBari, V.A., Needle, N.A.
and Bennun, A. Catalase as a
manganese-dependent NADS4PH peroxidase.
(1984). Biochemical Society Bulletin
vol. 6, No. 2, page 53 (609/32). Bennun, A., Needle, N.A.
and DeBari, V.A. Infrared spectroscopy
of erythrocyte plasma membranes. (1984). Biochemical
Society Bulletin, vol. 6, No. 2 (609/33). Sulner, J.W. and Bennun,
A. Ca 2+-dependent control of renin release. (1984). Biochemical Society Bulletin, vol. 6, No. 3, page 30 (610/29). Bennun, A., Seidler, N.
and DeBari, V.A. A model for the regulation of haemoglobin affinity for
oxygen. (1984). Biochemical Society
Bulletin, vol. 6, No. 3, page 30 (610/30). Sulner, J.W. and Bennun,
a. KCl-stimulated renin release.
(1984). Abstracts Book - N.Y. Acad. Sci., Second Colloquium in Biological Sciences, p-39. Bennun, a., Seidler, N.
and DeBari, V.A. Divalent metals in
the regulation of hemoglobin affinity for oxygen. (1984). Abstracts Book - N.Y. Acad. Sci., Second Colloquium in
Biological Sciences, p. 92. DeBari, V.A., Cruz, R.A.,
Needle, N.A., and Bennun, A.
Dinucleotide oxidation by the Fenton reaction. Sulner, J.W. and Bennun,
A. Acachidonic acid stimulation of renin release: Dependence upon calcium, calmodulin and
B-adrenergic agonism (1985). Federation Proceedings vol. 44, No. 4,
1127. Bennun, A. Dependence
Federation and Sulner, J.W. Tyramine
stimulation of renin release: upon calcium, calmodulin and prostaglandins. Serban, G. Foldes, C. and
Bennun, A. Electrometric studies in
human nails in vivo - 5th
International Symposium on Bioengineering and the 5km (8/29-9/2,
19895). Hyatt Regency Hotel, Cruz, Rafaela A., Billey,
D., Bennun, A. and DeBari, V.A. The
Fe(II) - catalyzed peroxidation of nicotinamide adenine dinucleotides.
(1986). The 14 th Annual Minority
Biomedical Research Support Symposium, by Sponsored Div. Research Res. -
National Institute of Health, New Orleans, Aprill6-20. NBRS Book of
Abstracts, page B-32 comm. 191* Cruz, R.A.*, Batista, V.
and Bennun, A. Integration of hormonal
and endogenous regulation of adenylate cyclase. The l4th Annual Minority Biomedical
Research Support Symposium, sponsored by Div. research Res. National
Institute of Health, Casciano, C. and Bennun,
A. Characterization of the
interrelationship of the secretory activities of gastric tissues. Annals Vicario, P. Saperstein,
R., Katzen, H. and Bennun,, A.
Metabolic interactions of insulin-activated receptor kinase. Annals DeBari, V.A., Needie,
N.A., Bennun, A. Catalase as a
manganese-dependent NADPH peroxidase. Vicario, P., Saperstein,
R., and Bennun, A. Divalent metal ion
interaction and activation of rat liver insulin receptor Tyrosine Kinase.
1987 Fed. Proc. 46, 2075 comm. 877. Casciano, C. and Bennun,
A. A comparison of two inhibitors of
H+K+ ATPase in gastric tissue. (1987).
Fed. proc. Vol. 46 No. 6, 2106, comm. 1054 Bennun, A. Hemoglobin as a model for a coupling
mechanism between regulatory and active sites. (1987). Fed. proc. 46, No. 6 2047, comm. 712 Cruz, R., Batista, V.M.,
and Bennun, A. the integration of
hormones, Mg2+ and Ca 2+ on the regulation of Adenplate Cyclase. NIR
Centennial MBRS - Marc Symposium. Batista, V.M., Cruz, R.
and Bennun, A. Divalent metals and
hormones in the control of Adenplate Cyclase activity. NIR Centennial NBRs - marc Symposium. Submitted.
Casciano, C. and Bennun,
A. A Characterization of Two Inhibitors of H+K+ATPase in Gastric Tissue.
623rd meeting of the Biochemical Society at the Vicario, P.P., Saperstein,
R. and Bennun, A. (1987) Regulation of
insulin receptor tyrosine kinase (IRTK) by activatory Me+2, MeATP
sybstrate and free ATP-4
. 623rd meeting of the
Biochemical Society at the Vicario, P.P., Saperstein,
R. and Bennun, A. (1987)
Interrelationships of peptide substrate and metal-ATP with insulin receptor
tyrosine kinase. 624th Meeting of the Biochemical Society at Vicario,P.P., Saperstein,
R. and Bennun, A. (1987) Kinetic
studies of the reaction catalyzed by rat liver insulin receptor tyrosine
kinase. 38th Annual Meeting
of the American Physiological Society Casciano, C. and Bennun
A. The Effect of Li + on the Secretion
of HCO3 in Rat Fundic Tissue. (1987). Biochemical Society Bulletin. Vol. 9, No. 3, 73, comm. 103. Vicario, P.P., Saperstein,
R. and Bennun, A. Metabolic feedback control of insulin receptor tyrosine
Kinase. (1988). The FASEB
Journal. Vol. 2, No. 6, A1758 comm.
8490. Casciano, C., Kaminska,
G.Z. and Bennun A. Protection of rat gastric mucosa by two inhibitors of H K
-ATPase against acid ethanol induced gastric lesions. (1988). The FASEB Journal. Vol. 2, No. 4, A617 comm. 1863. Casciano, C., Kaminska,
G.Z. and Bennun, A. (1988). Protection
of rat gastric mucosa nu omhonotprs of
H+K+ against acid-ethanol induced gastric lesions IUB-l4th International Congress of Biochemistry, Vicario, P.P., R.
Saperstein and A. Bennun. The interaction of rat liver insulin receptor
tyrosine kinase with heavy metals. (1988). 626th Meeting of the Biochemical Society Bulletin 10, No. 1, 150,
comm. 225. III.
PARTICIPATION IN SCIENTIFIC MEETINGS 8/7 – 8/12/66 -
Interaction between chloroplast particles and coupling factor. Latin American Association of physiological
Sciences Mar del Plata, 11/21 – 3/9
– 1967 – Interaction between
chloroplast particles and coupling. Federation American Societies Experimental
Biology, FASEB 6/1970 – Interaction of
the chloroplast coupling factor with protons and water. V Argentine Congress of Biological Sciences 6/24 – 1971 - Regulation of
heat-activated Ca2+-dependent ATPase. Biophysical Society 9/14 – 9/1972 - Bioenergetics of
chloroplast. N.A.T.O. Advanced study
institute on "Primary Molecular Events in Photobiology" 2/7 – 7/1973 - Hypothesis for a
biochemical memory pathway. International 1974 - The unitary
hypothesis and the relationship of enzyme modification to the pathophysiology
of membranes. American Society of Biological Chemist, Inc.
(Biochemistry/Biophysics) 9/2 – 9/6/74 - A model
mechanism for coupled phosphorylation.
American society of Plant Physiology (Third International Congress of
Photosynthesis) 1974 - Integration of
hormone-dependent effects on adenyl cyclase.
American Chemical Society (168th) annual meeting 12/9 – 12/12/74 -
Properties of fat cell's adenylate cyclase.
Biochemische Gessellschaft der DDR, Dresden Congress in biological
regulation (Invited lecturer) 1975 - Norepinephrine ad
the pathophysiology of neuronal membrane.
Biophysical Society annual meeting 1975
- A model pathway for coupled phosphorylation.
American Society of Plant Physiology. 1975 - Post-synthetic
stabilized modification of adenylate cyclase by metabolites. The Biochemical
Society (557th Meeting) 1975 - A metal
coordinative chemistry model for coupled phosphorylation. American Society
for Photobiology (annual meeting) 1975 - Relevance of the
alteration of thresholds of adenyl cyclase response. American Chemical
Society (l7Oth meeting) 11/1975 - Hormonal control
adenylate cyclase in lipolysis. International Union of Biochemistry (plenary
lecturer at 75th Symposium on the Regulation of Metabolic Pathways Bogota, 1976 - Regulation of
thresholds of adenylate cyclase response. Biophysical Society 1977 - A structure and
function model for coupling between regulatory and active site. Biophysical
Society 1978 - Metal and ATP
interactions with adenylate cyclase. National Meeting Biophysical Society, 1980 - The effect of
hormones on fat cell adenylate cyclase. Joint presentation with R. Cruz at
Comparative Endocrinology Conference of American Society of Zoologist, 1980 - Regulation of the
responsiveness to calcium of hypothalamic adenylate cyclase (with H.
Ohanian). ASBC/BS fleeting, New 5/22 – 24/84 - The effect
of hormones on metal and metal-ATP interactions with fat cell adenylate
cyclase. Corresponding co-presenter R. Cruz. Eastern Regional Conference on 2/13 – 16/83 - Transport
of guanosine 3', 5' cyclic monophosphate into human erythrocytes. Corresponding co-presenter V. DeBari. 27th Annual Meeting Biophysical Society 6/5 – 9/83 - Multiple internal
reflectance-infrared spectroscopy (MIR-IR) of erythrocytes plasma
membranes. Corresponding co-presenter
V. DeBari. 74th Annual Meeting American Soc. of Biological Chemists, 2/19 – 23/84 - Regulation
of the pentose phosphate pathway in the human erythrocyte Corresponding
co-presenter V. DeBari. 28th Annual Meeting Biophysical Society 6/84 - Catalase as a Mn2+
dependent NADPH erpxodase (609/32). Co-presenter V. DeBari. 609th Meeting of Biochemical Society, 6/84 - Infrared
spectroscopy of erythrocyte (609/33). Co-presenter, V. DeBari. Biochemical
Society, 8/84 - Ca2+ -
dependent control of renin release (610/29). 610 th Meeting Biochemical
Society, Stirling, 8/84 - A model for the
regulation of haemoglobin affinity for oxygen (610/30). Co-presenter N.
Scidler. 610th Meeting Biochemical
Society, 4/21 – 26/85 -
Dinucleotide oxidation by Fenton reaction (Comm. 2987). Co-presenter, V.
DeBari. 69th Annual Meeting FASEB, 4/21 – 26/85 - Arachidonic
acid stimulation of renin release (Comm.4191). Co-presenter, J.W. Sulner.
69th Annual Meeting FASEB, 4/21 – 26/85 - Tyramine
stimulation of renin release (Comm. 4192).
69th Annual Meeting F'ASEB, 8/31 – 9/86 - Session on
haemoglobin, catalytic functions.
Sept. IV. PROFESSIONAL ACTIVITIES A. INVITED
LECTURES 1959 - 1961 - Seminar Program,
Laboratory for Ceil Metabolism, National Atomic Energy Commission and 1961 - Department of
Biochemistry, 1962 - 1964
- Seminar Program, Section of Biochemistry, Weizmam
Institute of Sciences and Dept. of Molecular Biology, Neizmann Ist. of
Sciences. 1965 - Dept. of Physiology
and Pharmacology, Duke university, 1965 - Biochemistry
Section, Public Health Research Institute of the City of New York, Inc. 1967 - Seminar Program, Dept.
of Biochemistry and Molecular Biology, 1967 - Section of
Genetics, Development and Physiology, 02/67 - Charles F.
Kettering Research Laboratory, 1968 - Depart. of Biology,
1968 - Dept. of
Biochemistry, 1968 - Dept. of Chemistry,
1969 - Seminar Program,
Dept. of Zoology, NCAS, 7/70 - Section of
Biochemistry, Institute of Microbiology “Malbran” 7/70 - Dept. of Biological
Chemistry, 7/70 - Institute Campomar,
1/71 - Section of
Biochemistry, Agricultural & Experimental Station, 7/71 - Dept. of
Biochemistry, 6/71 - Dept. of
Biochemistry, New Jersey College of Medicine arid nentistry, 9/71 - Seminar Program
Dept. of Chemistry, NCAS, 5/72 - Dept. of Biology, 3/72 - The Institute for
Enzyme Research, University of 7/72 - Dept. of
Biochemistry, 1973 - Dept. of
Biochemistry, 7/73 - lecture at the
Zentralinstitut fur Molekularbiologie, Akademie der Wissenschaften der DDR,
Berlin Buch 1115, DDR 1974 - Zentralinstitut fur
7/74 - Dept. of
Biochemistry, 7/74 - Professional course
and workshop in Metabolic Regulation. Biochemical Association, 7/74 - Conference on
television 3/75 - Lecture award at
the Chemistry Institute, 7/77 - 12/77 - Biochemistry
Department, 12/77 - Lecture award at
the Biochemical Association, 12/78 - Department of Biological
chemistry, 12/78 - Department of
Biochemistry, 12/78 - Institute Campomar
of Medical Research as guest of Director Luis F. Leloir, Nobel Laureate 1/79 - Lecture presented
at Dept. of Biological Chemistry, School of Medicine, and Dept. of
Biochemistry, School of Pharmacy, University of Buenos Aires 7/80 - Differential
properties of adenylate cyclase from hypothalamus, corpus striatum and cortex.
A. Bennun. Seminar, Dept. of Physio. and Biophys., C.I.E.A., natl. Polytech.
Inst., 7/80 - Properties of fat
cell adenylate cyclase". A.
Bennun. Seminar, Dept. Physiol. and Biophys., C.I.E.A., Natl. Polytech.
Inst., 2/82 - Workshop in
adenylate cyclase. 2/82 - Role of ionic
equilibria in the control of adenylate cyclase activity. Department of
Biological Chemistry, School of Chemical Sciences, University of 3/82 - Dynamic controls of
metabolic pathways. Biochemical Society of 5/82 - Workshop in
interactions of coupling factors from chloroplasts with components of the
chloroplast membrane. Biochemistry Section, Weizmann Institute of Science, Rehovot 12/83 - Adenylate cyclase
- control mechanisms, workshop; 1/84 - Hexose cycle in the
erythroclyte, workshop, School of Pharmacy and Biochemistry University
of Buenos Aires 1/85 - “Thermodynamics of
hydrogen bondino and protein structure in hemoglobin and
deoxyhemoglobin", workshop in the Division of Physical Chemistry,
Department of Biological Chemistry, School of pharmacy and Biochemistry;
University of Buenos Aires 9/85 - “A model for the
regulation of hemoglobin affinity for oxygen" 9/86 - Department of
Biochemistry and molecular Genetics, Pasteur Institute, 9/85 - Divalent metals in
the regulation of hemoglobin affinity for oxygen:, Department of Structural
Immunology, Pasteur Institute, 9/85 - "Hemoglobin
structure and function", Biology Section, Curie Institute, 12/85 - Mechanism of
cooperativity and hemoglobin function", Division of Physical Chemistry,
School of Pharmacy and Biochemistry, University of Buenos Aires, Bs As. 12/85 - “Thermodynamics of
hemoglobin function", research Department National Institute Mario
Fatala Chaben, Ministry of Health and Social Action of Argentina, Bs As. B. MEMBERSHIPS TO THE EDITORIAL BOARDS OF SCIENTIFIC
JOURNALS 1975 – 1978 - Member of
the Editorial Board of “Science of Biology Journal" 1978 – 1983 - Member of
the Editorial Board of Journal of Bioenergetics and Biomembranes - Plenum
Press 1976 - Reviewer for
Biosystems 1979 - Reviewer for the Journal
Cancer Biochemistry Biophysics 1983 - Present Reviewer
for the Journal of Toxicology and Environmental Health 1983 - Present Member of
the Editorial Board of Journal of Toxicology and Environmental Health,
Hemisphere Publishing Corporation C. MEMBER OF THE FOLLOWING SCIENTIFIC SOCIETIES 1969 – 1982 - American
Chemical Society, Division of Biological Chemistry 1969 – Present - American
Society of Plant Physiology 1969 – 1981 - Genetics
Society of 1969 – 1974 - American
Nuclear Society, radioisotopes and education divisions 1970 to Present - Biophysical society, Bioenergetics group 1970 to present - New York
Academy of Sciences 1972 – 1974 - Federation
of American Scientists 1972 – 1981 - Argentine
Society of Biochemical Research (SAIR) and Pan American Association of
Biochemical Societies (PAABS) 1974 – 1978 - American
Society for Photobiology 1974 to present - The
Biochemical society arid Federation European Biochemical Societies (FEBS) 1976 – 1978 - Society for
Supramolecular Biology 1977 to present - American
Society of Biological Chemists, Inc. and Federation of American Societies for
Experimental Biology (FASEB) 1979 to present - American
society of Zoologists D. CHAIRING OF SCIENTIFIC CONFERENCES Chairman, Biochemistry
Platform Session 1, morning Annual Minority Biomedical Research Support
(NBRS) the Division of Research Resotirces of the National The Fairmont
Hotel, New Orleans, La., April 16-20, of April 17, the l4th Symposium,
sponsored by of Health, Institutes 1986. Chairman, Biochemistry
Platform Session 2, afternoon of April 17, the l4th,Amual Minority Biomedical
Research Support (KBRS) symposium sponsored by the Division of Research
Resources of the National Institutes of Health The Fairmont Hotel, New
Orleans, La., April 16-20, 1986. E. ATTENDANCE TO ADVANCE SCIENTIFIC STUDIES
INSTITUTES 1958 - 9/72 - N.A.T.O. Advanced Study Institute on
"Primary Molecular Events in Photobiology", F. PROFESSIONAL. SERVICE 1970 - Has acted as
reviewer for grant applications by request of the National Science Foundation 1973 - Served as National
Institute of Health supported consultant for the 1973 - Member of the
consultant organization of the Biophysical Society 1975 – 1976 - Member of
the committee on Bioenergetics of the International Union of Pure and Applied
Biophysics (IUPAB) 1981 - Consultant New
Jersey Schools Board Association 1982 - Evaluator, Committee
on an assessment of quality-related characteristics of Research-Doctorate
programs, National Research Council, In progress. 1977 - Member, Public
Health Panel. 1980 - Chairman, Public
Relations Committee. 1980 - Consultant NIH - In
progress 1983 - Participant in the
NIGNS, NIH funded “Visiting Scientists
for Minority Institutions Program”. Listed in roster of available scientists. G. MEMBER OF NON-ACADENIC INSTITUTIONS 1970 – 1972 - Sigma XI, 1975 - Smithsonian
Institution 1977 – 1979 - 1978 – 1979 -
International Platform Association 1978 – National IMAGE,
Inc. H. LISTED IN BIOGRAPHICAL PUBLICATIONS American Men of Science,
(1970) Bowker Co., 11th edition, supplement 6, p. 48, ACS Directory of
Graduate Research, (1975) International Scholars Directory,
International Scholarly publishers, first edition p. 17, 1972 Who's Who in the East,
Narquis Who's Who, Inc., 1Sth edition, p. 50, 1975, 1976 1978 - listing in special section of Who's who in
America Comunity leader:and Noteworthy American:, American Biographical
Institute, Bicentennial edition, pp. 69 & 70 1975, 1976 Dictionary of
International Biography, International Biographical Center, Cambridge,
England, p. 63, 1977 V.
GRANTS RECEIVED 11/65 – 11/66 - USPHS
Natl. Cancer Inst. (CA-87-66 supply allowance) Resolution and Reconstitution
of chloroplasts: $500 10/67 – 05/68 - University
of 09/68 – 08/71 - USPHS
Natl. Inst. Genl. Medical Sciences 1-Rol GM 16390-91 Resolution and
reconstitution of developing membranes: $18,860; 02: $10,798; 03: $15,885 09/69 – 08/79 - USPHS
Natl. Inst. Genl. Medical Sciences Resolution and reconstitution of
developing membranes: $15, 110 7/77 – Warner-Lambert Co. - Regulatory effects of hormones on adenylate
cyclase from rat's fat cells; Research materials $16,000 10/77 – Mallinckrodt
Nuclear - Research materials $2,500 8/69 – 8/77 - Research
Corporation - Characterization of the functional properties of a structural
resolved factor by the effect of its antiserum on photophosphorylation $8,000 11/75 – International
Union of Biochemistry - Travel grant to 7Sth International 1984 – 1986 -
Bristo-Myer: Estimated value -
equipment $3,000; supplies $1,000 1985 – 1986 - Schering
Plough Company - Estimated value - supplies $800 1986 -
Hoffmann-LaRoche: Estimated value
equipment $18,200; supplies $1000 1984 - Develop contact, preliminary grant application, acted as Program
coordinator for the initial NIH visitation and coordinator for the research
units of Rutgers – 07/84 – 06/85 - 06/85 – 06/86 - 06/86 – 06/87 -
Independent Associate Investigator MBRSP (5S06RR08228) College research
award $13,394 (Principal
Investigator: Dean of Graduate
School-Newark whose 0ff ice administers the grant for the independent
research associate investigator) 1982 Allocation: Student
$1,500; supplies - $500 1983 Allocation: Student
$1,500; supplies - $500 1984 Allocation: Student
$1,500; supplies - $500 1985 Allocation: Student
$1,500; supplies - $500 1986 Allocation: Student
$1,500; supplies - $300 Biomedical Sciences
Support Grant: USPHS FR7059: 07/69 – 05/70 - Resolution
and reconstitution of biomembranes $1,0OO 06/70 – 05/71 - Resolution
and reconstitution of biomembranes $400 06/71 – 05/72 - Resolution
and reconstitution of biomembranes $1,000 06/72 – 05/73 -
Interaction between coupling factor 1 arid ligands arid their relevance to membrane phenomena $1,000 06/73 – 05/74 - Hormonal-dependent
modification of adenylate cyclase $800 06/75 – 05/76 - Hormonal -
dependent modification of adenylate cyclase $600 04/76 – 03/77 - Hormonal
and metabolic control of adenylate cyclase in rat's brain $600 04/77 – 03/78 - Hormonal
regulation of adenylate cyclase $600 04/78 – 03/79 - Hormonal
regulation of adenylate cyclase $600 04/79 – 03/80 - Hormonal
regulation of adenylate cyclase $800 04/80 – 03/81 - Hormonal
regulation of adenylate cyclase $800 04/81 – 03/82 - Hormonal
regulation of adenylate cyclase $800 04/82 – 03/87 - Regulation
of renin release $500 every year 06/89 – 06/70 - Resolution
and reconstitution of biomembranes $2,500 07/70 – 06/71 - Travel
grant Photobiology Symposium of the V° of Biological Sciences $500 08/72 – 05/73 - Travel
grant $500 12/72 – 05/73 - Physio-chemical
forces that result in modification of enzyme activity $1,000 06/73 – 05/74 -
Interaction between coupling factor and ligands $750 06/77 – 05/78 - Hormonal
control of rat's fat cells adenylate cyclase $500 06/81 – 05/82 - The Effect
of hormones on adipocyte adenylate cyclase $500 Charles and Johanna Busch Memorial Fund 1974 – 1975 - The role of
enzyme modification in the friction and panthophysiology of membranes $6, 500 09/74 – 11/74 Visiting
scientist under the Academic Leave Program of Rutgers University at the
Laboratoire d'Enzimologie Centre National de FASP Grants Committee 07/78 – 12/78 Hormonal regulation adenylate cyclase
system $500 Grants to Obtain Grants 04/78 – For general
support $260 SCHOLARLY/CREATIVE ACTIVITIES VI.
TEACHING 1957 - Research
technology, non-credit course, Dept. of histology and Embryology, 1958 - Sections of
Experimental Biochemistry, Dept. of Biochemistry, 1958 – 1962 - Sections of
Biological Chemistry, 1967 - 1969 - Biology
103-104, General Biology, Dept. of Biology, 1967 - 1969 - Biology 379,
1968 - 1969 - Biology 354,
Cellular Biochemistry, Dept. of Biology 1968 - 1969 - Biology 257,
Plant Physiology, Dept. of Biology, 990:494
Seminar in Zoology (1 cr) 115:413
Experimental Biochemistry (2 cr) 990:411
Endocrinology 115:414
Experimental Biochemistry (2cr) 990:204
Physiological Zoology (4 cr) 120:101 General
Biology Laboratory 115:310 Elementary
Biochemistry 115:312 Elementary Biochemistry 115:404 General
Biochemistry 760:407 General
Physiology 115:405 Problems in
Biochemistry 990:491 Special
Problems in Zoology Courses Taught (at 115:513 Metabolic
Pathways Controls (3 cr) 990:513 Metabolic
Pathways Controls (3 cr) 115:514 Metabolic
Pathways Mechanisms (3 cr) 990:514 Metabolic
Pathways Mechanisms (3 cr) 990:511 Advanced
Endocrinology (3 cr) 990:616 Special
Topics in Biochemistry (1 cr) 115:606 Advanced
Studies in Biochemistry 115:701 Research in
Zoology 990:509 Advanced
Problems in Zoology 160:572
Biochemistry Supervision of Honors
Thesis Completed by Students in the Undergraduate Honors Program: 1969
– 1968 - W.
Velez-Vega and M. Dieppa - Honors Program Advisor - 1981 - Nancee A. Novak -
The effect of cyclic nucleotides upon glycolysis in the human erythrocyte 1982 - Bessie B. Fouces -
Kinetic studies on brain adenylate cyclase 1984 - Sylvia Menendez -
The role of energy charge in the regulation of brain adenylate cyclase
activity Summer Supervision of 1982 Patricia Grady 1983 Patricia Grady 1984 James Anderson 1986 Manuel Dasilva VII. UNIVERSITY SERVICE 1967 – 1969 - Member of
the Graduate Faculty, Department of Biology, 1970 - Full member of the
graduate School of Rutgers University. 1970 - Full member of the cooperative
Graduate Program in Biochemistry, 1970 – 1973 - Member of
the University Science Council, 1975 - . Member of the
Academic Standards Committee of the Cooperative Graduate Program of
Biochemistry. 1970 - Member of several
graduate students’ committees in the Graduate Program in Biochemistry 1970 - Full member
Graduate Program in Zoology. NCAS 1970 - Member of several
graduate students committees in the
Graduate Program in 1975 – 1978 - Member of
the NCAS Academic Affairs Committee 1977 – 1978 - Member of 1979 – 1981 - Member of 1977 – 1978 - Member of
Curriculum Committee, Dept. of Zoology and 1978 - Member of Executive
Committee of the Graduate Program in 1979 - Member of Teaching
Evaluation Committee NCAS 1978 - Member , Cancer
Coordinating Committee of Rutgers, 1983, 1985 - Member, Executive Committee Graduate Program in 1984 – 1986 - Member of the advisory committee for MBRS program in
Newark Campus of Rutgers. VIII. GRADUATE STUDENT SUPERVISION Supervision of Ph.D. candidates
research and titles of Ph.D. dissertations completed by students under my direction and initial
post-doctoral positions: Susan B. Golz - Regulation
of brain adenylate cyclase by physiological and pharmacological factors. Ph.D. in Zoology awarded May 1976. Initial Position: Assistant
Professor, Robert H. Harris -
Hormonal regulation of rat fat cell CAMP levels and fat ceil membrane
adenylate cyclase. Ph.D. in
Biochemistry awarded November 1977. Initial Position: Research specialist,
Division of Metabolism of Food and Drug Administration, Heripsime Ohanian - The
role of divalent metals, adenosine 5'-triphosphate and hormones in the
regulation of rat brain adenylate cyclase.
Ph.D. in Zoology awarded June 1980.
Initial Position: Vincent A. DeBari -
Regulation of the hexose monophosphate pathway in cyclic nucleotides and
divalent cations in the human erythrocyte. Ph.D. in Zoology awarded May 1981.
Initial Position: Director, Renal
laboratory, Joseph W. Sulner - Renal
renin release - a stimulatory role for Calcium Ph.D. in Zoology awarded May
1984. Postdoctoral Hoffman – Laroche. Initial Position: Associate
Investigator N.Y. Veteran's Hospital. Rafaela Cruz - The
integration of hormones, Ng 2+ Ca2+ and their
nucleotide complexes on the regulation of brain adenylate cyclase. Ph.D. in
Zoology awarded October 1986. Initial
Position: Adjunct Faculty in Anatomy and Physiology at Pasquale Vicario – The
regulation of Insulin Receptor Tyrosine Kinase by Insulin, Divalent metal
cations and metal – ATP substrate. Ph. D. in Zoology awarded May 1988. Initial Position: Investigator at
Merck Company. Christopher Casciano – The
pharmacological characterization of the bicarbonate, proton, and mucus
transport systems of the rodent gastric mucosa and their significance in
cytoprotection. Ph. D. in Zoology awarded September 1988. Initial Position: Investigator at
Schering Company. Supervision of Ph.D. candidates research under my
direction: 1984 G. Serban 1985 P. Uychich 1985 L. Rumennick Supervision of Masters candidates research and Masters Thesis
completed by students: 1975 - Chairman of the
committee for the Master in Zoology of Susan Brydon Golz. M. Sc. awarded
1975. 1978 - Chairman of the
committee for the Master in Zoology of Heripsime Ohanian. M. Sc. awarded
1978. 1981 - Chairman of the
committee for the Master in Zoology of Rafaela Cruz. M. Sc. awarded 1981 Supervision of research: 1968 – 1969 - Advisor in
the Biology Program to 1. Bulla, M.S. candidate at 1970 – 1971 - Advisor in
the Graduate Program of Biochemistry to E. Albanese and V. Cannistraro. 1970 – 1971 - Advisor in
the Master in Zoology Sciences Program to E. Wallendjack 1977 - Research advisor to
Kamran Borhanian, student at NJCMD, 1977
- Postdoctoral research advisor to Salette de Farias
on a leave from Institute Butantan, 1984 - Research advisor to
Norbert Seidler, student at NJCMD, Membership on Graduate Student Committees: 1974 - Vincent Rama
(Ph.D., Zoology) 1977 - Jeff Stevens (M.S.,
Biochemistry) 1980 - Jeff Stevens
(Ph.D., Biochemistry) 1979 - Rosy Jordan (M.S., Zoology) 1979 - Gerald Costa (M.S.,
Zoology) 1980 - Robert Jonathan
Weiss (M.S., Zoology) 1980 - Beryl Joyce Carby
(M.S., Zoology) 1980 - Vera Minak (M.S.,
Zoology) 1980 - Frank R. Settineri
(M.S., Zoology) 1981 - Diane G. Verga (M.S., Zoology) 1983 - Dolores Curtis (M.S., Zoology) 1983 - Marlene Blanco (M.S., Zoology) 1983 - Mark Schachman
(M.S., Zoology) 1983 - Thomas Brankner
(M.S., Zoology) 1984 - Lee D. Carpe
(Ph.D., Zoology) 1984 - Dennis Guiliani
(Ph.D., Zoology) 1985 - Eva-Pia Reich
(Ph.D. Zoology) 1985 - Ilda D. D'Onofrio
(Ph.D. Zoology) 1988 - John C. Anthes (Ph.
D. Zoology) IX. RESEARCH ACCOMPLISHMENTS A. Active Transport and
metabolic control a) It was demonstrated that
active transport of phosphate by yeast is directly dependent on high levels
of energy – rich phosphates maintained by either endogenous oxidation or by
exogenous substrates during oxidation by either glycolysis or respiration. b) It was shown that yeast’s
uptale of acetate, pyruvate, etc. and their oxidation rate within the Krebs
cycle, rather than under direct control by oxygen itself, is subject to an
energy dependent regulatory mechanism supported by ATP levels and their
equilibria with other energy rich phosphate compounds. B. Mechanism of oxidative phosporylation In 1963, the DNP –
activated, Mg – dependent ATPase of yeast was for the first time isolated and
characterized for its role in oxidative phosporylation. III. Mechanisms of enzymatic induction and reversion as cells adaptative
response to environmental changes. It was found that
Wuglena controls endogenous phosphatase levels by a low phosphate contration
– dependent C. Mechanism of
photophosphorylation a)
In 1964, the proposition
that high energy intermediates should be equated with conformational changes
was published. It was proposed that conformational change participated in energy conservation and
transduction. Ca2+ and cysteine wiche eleicit light – requiring
ATPases were shown to, modify the kinetic behavior of the ATP synthetase. ADP
and GDP show inhibitory constants (Ki) for the light – requiring ATPases of
the same numerical value that their Kms for residual and modified
phtophosphorylation. This would suggest the participation of a single, but
modigiable, active center in the mechanism which would determine the
direction of the ATP synthetase activity b)
The first successful
resolution and reconstitution of an energy – dependent ATPase function was
achieved for chloroplast light – requiring ATPases. Isolation and
purification of the ATP-synthetase choroplast’s coupling factor. It was also
shown that the latter becomes an ATPase by heat or trypsin treatmen. c)
Reso1ution and
reconstitution of the functlon for metal-dependent binding of the coupllng factor
(ATP synthetase-ATPase) by the chloroplast membrane. Characterization of
allotopic properties of the system.
Characterization of the Inhibitory capacity by the membrane on the
ATPase activity during binding. d)
The finding of the
capability of the ATPase to maintain cooperative interaction with 2 ATP, 4PO4,
2 ADP and 16 molecules of water, was used to postulate that the active form
of the ATPase is a structure characterized by conserving the energy of 14
H-bonds or 14 Ki1oca1ories (1 kilocaloráe per each solvating water molecule),
where as that of the inhibited form of the ATPase is a estructure
characterized by a decrease in energy content of the same value. This appears
to indicate the potential of the ATPase itself to function as a high-energy
intermediate during the turnover of the ATP synthetase system. e)
First report on the
allosteric kinetics of the ATPase (activated coupllng factor), and
demonstration that proton concentration has the capability to requlate the
modification of the ATPase from a form of high affinity for ATP and low
affinity for phosphate and ADP, into another form of opposite properties. D.
Energy Transduction a)
Kinetic and thermodynamic
parameters were described for the operatien of ATPase coupled for ionic
transport, muscle contraction, interaction between cytochromes and coupling
factor (ATPase) and the effect of ions in the modification of chloroplast’s
light-dependent activities. b)
The postulation of energy
transduction mechanisms resulting from the exergonic association of proteins
with divalent metals (Me2+). The turnover of the protein te a
dissociated state (endergonic) is driven by the chelation of the binding Me2+
by ATP4- (exergonic). ATPase activiy turns ATP4- into
the waker chelating agent ADP3- allowing Me2+ dissociation
and the return te the initial high concentratien of free Me2+. c)
The binding of a divalent
meta1 requirement for the EDTA-treated membranes binding of the coupling
factor-ATpase led to the postulation that electron transfer is coupled to
phosphorylation by cytochreine-dependent or pH-dependent modificatien of the
meta1 chelated states of R-groups located in the coupling site of the ATPase. d)
Consequently, the unitary
hypothesis can be differentiated from the chemical hypothesis in that it
postulates the formation of high energy intermediates by the transfer of
groups across coordinative bonds rather than across covalent bonds. It also
integrates conformational and chemiosmotic prepositions within a single
framework. Accordingly, the hypothesis
uses a single thermodynamic and kinetic framework for findings that were
previously explained only through the alternate use of three different
hypothesis. E. Hemoglobin It has been previously proposed that in an
open thermodynamic system, the turnover of the state of Me2+ as
ligand of pretein(s) would yield the energy of activation for driving
conformational and functional changes of protein. Accordingly, it was proposed that
oxygenation of Hb would lead Me2+ induce-fit a chelating
configuration of side chains. This binding of Me2+ to Hb would
dislocate proxima1 histidines from the positions reguired for steric
hindrance and would allow Bohr side chanin(s) te release their
proton(s). Mg2+ rather than
Zn2+, was suggested as the main physiological Me2+ to
compete with Zn2+ at cellular concentrations of 11 ug/g red cells
for the chelating side-chains of Hb. Oxygenation induces binding of Mg2+
or Zn2+ to Hb. Hence,
according to the mode1 the PH-dependent stability order of the chelated
complexes during deoxygenation would be: (Zn2+)2-oxyHb>(Zn2+,
Mg2+)-oxyHb>(Mg2+)2-oxyHb F.
Characterization of the hormonal and metabolic parameters that control
adenylate cyclase activation a)
GH potentiates the effect
of epinephrine, glucagon and theophylline increasing endogeneus cAMP levels
in fat celis. b) Isolated membranes show that epinephrine activates adenylate cyclase
by decreasing its Km for ATP and its Km for Mg2+ to reach a
maximal activatory effect. Adenylate cyclase can be described as a
receptor-coupled enzyme system in where the hormone binding by the receptor
increases the affinity of the enzyme's regulatory site for Mg2+.
The regulatory site also functions in the absence of epinephrine because high
Mg2+ concentration is also capable of activating adenylate cyclase
(19) G.
Characterization of hormonal and metabolic parameters that desensitize and
inactivate adenylate cyclase a)
It was found that an
adenylate cyclase membrane preparation from rat's cerebral cortex, during
prolongued preincunation at b) Solving multiple-equilibirum equations demonstrated that the
relationship between energy charge (ATP vs. ADP and AMP) and relative
concentration of free vs. chelated by nucleotides divalent metals (Me: Mg2+,
Mn2+, Ca2+) plas a modulatory role for adenylate
cyclase and insulin-dependent tyrosine kinase at three sites (substrate site,
Me2+-dependet regulatory site and Me-requiring site for control of
hormone responsiveness) H. Metabolic controls The cellular uptake of
glucose and/or the lysis of glycogen it is expected to change the
concentrations of chelating metabolites (sugar phosphates) and therefore the
relative concentrations of free versus chelated divalent metals. Thus a decrease in chelating metabolites
constitutes an amplification mechanism of the adenylate cyclase (AC)
response, since it simultaneously increases the concentrations of the
substrate (MgATP) and the positive modulator (Mg +2) and decreases
that of the inhibitor (ATP 4-).
Mg2+ or Mn2+, in contrast with Ca2+,
may control not only the basal activity, but also the hormonal responsiveness
of AC. A more comprehensive description of metabolic shifts requires the
examination of hormonal action and metabolic feedback not only on AC, but
also on insulin receptor tyrosine kinase (IRTK). The metal dependence of IRTK, as well as
the effect of changes on chelated species of ATP on this dependence, were
investigated to elucidate the integrative parameters which allow coordinated hormonal,
ionic and energy-charge control of the metabolic shift from glycolysis to
gluconeogenesis. I. Control mechanisms for secretory processes: a) Renin release in kidney
tissue. b) HCl versus bicarbonate
in gastric tissue MAJOR
RESEARCH INTERESTS Metabolic Regulation: in yeast
Energy transduction: in chloroplast and mitochondrial membranes, resolution
and reconstitution, ATAase Control mechanisms of
enzyme-membrane systems: hormone receptors-adenylate cyclase systems in fat
cells and brain membranes, insulin receptor tyrosinekinases in liver. Modeling of Energy transduction
mechanism: Hemoglobin Areas of Specialization -
Cellular actions of hormones Enzyme kinetics Cellular structure and function
Membrane biochemistry of organelles, chloroplasts and mitochondria |
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