ALFRED BENNUN, PH.D.

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II.  RESEARCH - PUBLICATIONS

A. JOURNAL ARTICLES (REFEREED, BIOCHEM.  SOC.  TRANS. BY EDITOR)

Stoppain, A.O.M., Bennun, A., and dePahn, E.M. Energy requirement for the anaerobic oxidation of acetate in baker's yeast. (1964). Biochim. Biophys. Acta 92, 176-178.

Bennun, A., dePahn, E.M., and Stoppani, A.O.M. Sorne properties of particle-bound intracellular ATPase (adenosinetriphosphatase) from baker's yeast.  (1964). Biochim. Biophys. Acta 89, 532-539.

Stoppani, A.O.M., Bennun, A., Effect of 2-4 Dinitrophenol on Krebs cycle and phosphate metabolism in baker’s yeast. Arch. Biochem. Biophys. 108, 258-265.

Bennun, Alfredo and Avron, Mordhay. Light-dependent and light-triggered adenosinetriphosphatases in chloroplasts. (1964). Biochim. Biophys. Acta 19, 646-648.

Bennun, Alfredo and Avron, Mordhay (1965).  The relation of the light-dependent and light-triggered adenosinetriphosphatases to photophos-phorylation.  Biochim. Biophys. Acta 109, 117-127.

Bennun, A. and Blum, J.J. Properties of the induced acid phosphatase and of the constitutive acid phosphatase of Euglena. (1966). Biochim. Biophys. Acta 125, 106-123.

Bennun, A. and Racker, E. Partial resolution of the enzymes catalyzing photophosphorylation IV.  Interaction of coupling factor I from chloroplast with components of the chloroplast membrane. (1969). J. Biol. Chem. 244, 1325-1331.

Bennun, A. Hypothesis for coupling energy transduction with ATP synthesis or ATP hydrolysis (1971). Nature (New Biology) 233, 5-8.

Bennun, A.  The unitary hypothesis on the coupling of energy transduction and its relevance to the modeling of mechanism. In "Mechanism of Energy Transduction in Biological System" (D.E. Green, ed.). (1974). Annals New York Academy of Sciences, 227, 116-145.

Brydon-Golz, S. and Bennun, A. Postsynthetic stabilized modification of adenylate cyclase by metabolites. (1975). Biochem. Soc. Trans. 3, 721-724.

Bennun, A. hypothesis on the role of liganded states of proteins in energy transducing systems.  (1975) Biosytems 7, 230-244.

Harris, R. and Bennun, A.  Hormonal control of fat cells adenylate cyclase (1976).  Molecular and Cellular Biochemistry 13, 141-146.

Brydon-Golz, S., Ohanian, H. and Bennun, A. Effects of noradrenaline on the activation and the stability of brain adenylate cyclase (1977). The Biochemical Journal 166, 473-483.

Ohanina, H., Borhanian, K. and Bennun, A.  The effect of manganese on the regulation of brain adenylate cyclase by magnesium and ATP. (1978) Biochem. Soc. Trans. 6, 1179-1182.

Harris, R.H., Cruz, R. and Bennun, A.  The effect of hormones on metal and metal-ATP interactions with fat cell adenylate cyclase.  (1979). Biosystems 11, 29-46.

Ohanian, H., Borhanian, K., deFarias, S. and Bennun, A.  A model for the regulation of brain adenylate cyclase by ionic equilibria  (1981). Special Issue of Journal of Bioenergetics and Biomembranes  Vol. 13, Nos. 5/6, 317-355.

DeBari, V.A., and Bennun, A.  Cyclic GNP in the human erythrocyte. Intracellular levels and transport in normal subjects and chronic hemodialysis patients.  (1982) Clinical Biochemistry 15, 219-221.

DeBari, V.A., Novak, N.A. and Bennun, A.  Cyclic nucleotide metabolism in the human erythrocyte (1984).  Clin. Physiol. Biochem. 2, 227-238.

Novembre, P., Nicotra, J., DeBari, V.A., Needle, N.A., and Bennun, A. Erythrocyte transport of cyclic nucleotides (1984).  Annals of the New York Academy of Sciences 435, 190-194.

Bennun, A., Needie, N.A., and DeBari, V.A   Stimulation of the hexose monophosphate pathway in the human  by Mn+2:  Evidence for a Mn – dependent  NADPH peroxidase activity. (1985). Biochemical Medicine. Vol. 33, 17-21

DeBari, V.A., Needie, N.A., and Bennun, A. Catalase as a manganese-dependent NADPH peroxidase. (1985). Biochem. Soc. Trans. 13, 125-127.

Bennun, A., Needie, N.A. and DeBari, V.A.  Infrared spectroscopy of erythrocyte plasma membranes (1985). Biochem. Soc. Trans. 13, 127-128.

Sulner, J.W. and Bennun, A.  Ca 2+ - dependent control of renin release (1985).  Biochem. Soc. Trans. 13, 363-364.

Bennun, A., Seidler, N. and DeBari, V.A.  A model for the regulation of haemoglobin affinity for oxygen (1985). Biochem. Soc. Trans. 13, 364-366.

Emma, J.E., Cervoni, P., Sulner, J.W. and Bennun, A. KCI-stimulated renin release (1986).  Annals of the New York Academy of Science, 463, 281-283.

Bennun, A., Seidler, N. and DeBari, V.A.  Divalent metals in the regulation of hemoglobin affinity for oxygen (1986).  Annals of the New York Academy of Science, 463, 76-79.

Bennun, A.  A coupling mechanism to interrelate regulatory with haemohaem interactions of haemoglonin. (1987).  Biomed. Biochem. Acta. 46, No. 2/3, 314-319.

Casciano, C. and Bennun, A. A characterization of two inhibitors of H+, K+ - ATPase in gastric tissue.  (1988). Biochem. Soc. Trans. 16, 27-29.

Vicario, P.P., Saperstein, R. arid Bennun, A. Regulation of insulin receptor tyrosine kinase by divalent metal cations, metal-ATP substrate and free ATP -4. (1988). Biochem. Soc. Trans. 16, 40-42.

Vicario, P.P., Saperstein, R., Katzen, H. and Bennun, A.  Metabolic interactions of insulin-activated receptor tyrosine kinase.  (1988). Annals New York Academy of Sciences 529, 92-95. 

Casciano, C. and Bennun, A.  Characterization of the interrelationship of the secretory activities of gastric tissue.  (1988)  Annals New York Academy of Sciences 529, 246-249.

Vicario, P.P., Sapertein, R. and Bennun, A. Role of divalent metals in the kinetic mechanism of insulin receptor Tyrosine Kinase. (1988). Arch. Biochem. Biophys. 261, 336-345.

Vicario, P.P., Saperstein, R. and Bennun, A. Role of Divalent Metals in the activation and regulation of insulin receptor tyrosine kinase. (1988). BioSystems 22, 55-56.

Vicario, P.P., Saperstein, R. and Bennun, A.  Interrelationships of peptide substrate and metal-ATP with insulin receptor tyrosine kinase. (1988). Biochem. Soc. Trans. 16.

Vicario, P.P., Saperstein, R. and Bennun, A.  Interactions of Insulin receptor tyrosine kinase with heavy metals. (1988). Biochem. Soc. Trans. 16,  896-897.

Vicario, P.P., Saperstein, R. and Bennun, A. Interaction of MnATP and peptide substrate with insulin receptor tyrosine kinase. (1989). Biochem. Soc. Trans. 17, 1108-1109.

Vicario, P.P. and Bennun, A. Regulation of insulin receptor tyrosine kinase by metabolic intermediates. (1989). Biochem. Soc. Trans. 17, 1110-1111.

Casciano, C. and Bennun, A.  The effect of Li + on the secretion of HCO3- in Rat fundic tissue. (1989). Biochem. Soc. Trans. 17, 1111-1112.

Casciano, C., Kaminska, G.Z., and Bennun, A. Investigations on the Cytoprotective Mechanisms of Thiocyanate in Rat Gastric Mucosa. (1989). Biochem. Soc. Trans 17, 1113-1114.

Vicario, P.P. and Bennun, A. Separate effects of Mg2+, MgATP, and ATP4- on the kinetic mechanism for insulin receptor tyrosine kinase. (1990). Archives of Biochem. and Biophys. Vol. 278, 99-105.

Sulner, J.W., Emma, J.E. and Cervoni, P., Bennun, A. KCL stimulation of In Vitro renin release:  Prostaglandin and Noradrenergic components.

 

B. CHAPTERS IN BOOKS, REVIEN ARTICLES AND PROCEEDINGS

Stoppani, A.O.M., Ramos, E.H., Widuczynski, I., Bennun, A., and De Pahn, E.M.  The effect of 2,4 - dinitrophenol on the oxidation of endogenous and exogenous substrates by the yeast Saccharomyces cerevisiae (1962).  Proc. Conf., Mexico City, 1961 in "Use of Radioisotopes in Animal Biology and the Medical Sciences" (C.M. Fried et al., eds.)  Vol. 1, pp. 241-252, Academic Press, London.

Stoppani, A.O.M., Bennun, A., and De Pahn, E.M.  Effect of DNP on the metabolism of phosphates in Saccharomyces cerevisiae (1965).  Symp. of the inter-Am. Nuclear Energy Commission, Valparaiso, 1964, in "5th inter-American Symposium on the Peaceful Application of Nuclear Energy."  J.D. Perkinson, and the Secretariat of IANEC, pp. 59-68, Organization of American States, Washington, D.C.

Bennun, A.  Interaction of the chloroplast coupling factor with protons and water (1971).  Congreso Argentino de Ciencias Biologicas, 1970 in "Recientes adelantos en Biología" (J.A. Moguilllevsky and R. Mejía, eds.)  pp. 254-264, Univ. of Buenos Aires Press.

Bennun, A.  Properties of chloroplast's coupling factor-1 and a hypothesis for a mechanism of energy transduction (1971). Proceedings First European Biophysics Congress, Baden, Austria, 1971.  In “ Photosynthesis, Bioenergetics, Regulation, Origin of Life” (E. Broda, A. Locker, and H. sprínger-Lederer, eds.)  Vol. IV. pp. 85-91, Wiener Medizinischen Akademíe, Vienna.

Bennun, A. and Bennun, N.  Hypothesis for a mechanism of energy of energy transduction.  Sigmoidal kinetics of chloroplast's heat-activated ATPase.  (1972) In Proc 2nd Int Cong. On Photosynthesis Res. (Giorgio Fortí, Mordhay Avron and, Andrea Melardri, eds.) Vol. 2, 1115-1124, Dr. W. Junk N.V. Pub.,  The Hague.

Bennun, A.  A model mechanism for coupled phosphorylation.  (1974) Proc. 3rd Int. Cong.  Photosynthesis, Rehovoth (M.Avron, ed.) Vol. 2 pp. 1107-1120 Elsevier Sci. Pub. Co. Amsterdam.


C. PUBLICATIONS IN RAPID – COMMUNICATION JOURNALS

Bennun, A.  Hypothesis relating an ion-stimulated ATPase to the molecule code theory of memory. (1969). Bioenergetics Bulletin 1, No. 6, 49-50.

Bennun, A.  Effect of pH on heat-activated, Ca2+-dependent ATPase. (1970). Bioenergetics Bulletin 2, No. 7, 63-65.

Bennun, a.  Hypothesis for a mechanism of energy transduction. (1971). Bioenergetics Bulletin 3, No. 2, 11-15.

 

D. COMMUNICATIONS AND ABSTRACTS

Bennun, A. Phosphorus metabolism and its relation to the activity of adenosínetríphosphatase in Baker's yeast (Saccharomyces cerevisiae). (1965). Dissertation Abst.  Vol. XXVI (2), 665-6.

Bennun, A. and Racker, E.  Interaction between chloroplast particles and coupling factor. (1966). VII Cong. A.L.A.C.F., 7-12 Aug. 1966, Mar del Plata, Arg., Acta Physiol. Latino Americana, Vol. XVI, Suppl. No. 1, 16.

Bennun, A. and Racker, E.  Interaction between chloroplast particles and coupling factor. (1967).  Federation Proc., Vol. 26-2 609.

Bennun, a. and Blum, J.J.  Properties of the induced phosphatase and of the constitutive acid phosphate of Euglena. (1966). B.B.A. 65466.

Bennun, A. and Bennun, N. Regulation of heat-activated ca2+-dependent ATPase, l5th Annual Meeting Biophysical Society Abstracts, 39a, 1971.

Bennun, A.  The unitary hypothesis for coupling of energy transduction and its relevance to the modeling of mechanisms for ATP synthesis or ATP hydrolysis. Conference on the Mechanism of Energy Transduction in Biological Systems Abst.  The New York Academy of Sciences February 7 - 9,  1973.

Bennun, A.  Hypothesis for a biochemical memory pathway IUB. (1973). Ninth International Congress of Biochemistry, Abstract book Dc 27, 459, Stockholm.

Bennun, A.  The unitary hypothesis and the relationship of enzyme modification to the pathophysiology of membranes. (1974).  Biochemistry / Biophysics 1974 Meeting Abstracts, Fed. Proc. 33, 1528.

Bennun, A. A model mechanism for coupled phosphorylation. (1974). Abstracts book. 3rd International Congress on Photosynthesis, Rehovoth.

Harris, R.H. and Bennun, A.  Integration of hormone-dependent effects of adenyl cyclase. (1974). Abstr. Am. Chem. Soc. 168th Meeting Biol. 130

Bennun, A. and Brydon-Golz, 5. Norepinephrine and the pathophysiology of neuronal membranes.  (1975). Biophysical Journal 15, No. 2, 49a.

Bennun, A.  A model pathway for coupled phosphorylation. (1975). Plant Physiology 56, No. 2, 47.

Brydon-Golz, 5. and Bennun, A. Post-synthetic stabilized modification of adenylate cyclase by metabolites. (1975). The Biochemical Society Agenda Papers, 557th Meeting, Liverpool, pp. 27.

Bennun, A. A metal coordinative chemistry model for coupled phosphorylation. (1975). Abstracts 3rd Annual Meeting American Society for Photobiology, pp. 79.

Brydon-Golz, S. and Bennun, A. Relevance of the alteration of thresholds of adenylcyclase response. (1975). Abstr. papers Am. Chem. Soc. 17Oth Meeting, Biol. Chem., 165.

Brydon-Golz, S. and Bennun, A. Regulation of thresholds of adenylcyclase response. (1976). Biophysical Journal 16, No. 2 part 2, 190a.

Bennun, A. A structure and function model for coupling between regulatory and active site. (1977). Biophysical Journal 17, 239a.

Harris, R., Cruz, R. and Bennun, A. Metal and ATP interactions with adenylate cyclase. (1978). Biophysical Journal 21, 14a.

Harris, R., Cruz, R. and Bennun, A.  Calcium and calcium-ATP interactions with adenylate cyclase. (1978). Federation Proc. 37, 1789.

Harris, R. and Bennun, A. Hormonal control of fat cells adenylate cyclase. (1978). Feeding, Weight and Obesity Abstracts, 2w1055.

Ohanian, H., Borhanian, K. and Bennun, A.  The effect of the equilibrium concentrations of metal and ATP on adenylate cyclase.  (1978). Abstr. Am. Chem. Soc. 176th National Meeting Biol. 51.

Ohanian, H., Borhanian, K. and Bennun, A.  The effect of manganese on the regulation of brain adenylate cyclase by magnesium and ATP.  (1978) The Biochemical Society Agenda Papers 57th meeting, Oxford.

Ohanian, H. and Bennun, A. Regulation of the responsiveness to calcium of hypothalamic adenylate cyclase.  (1980). Fed. Proc. 39 (6) 2106.

Harris, R.H., Cruz, R. A. and Bennun, A.  The effects of hormones on metal and metal-ATP  interaction with fat cell adenylate cyclase.  Eastern Regional Conference on Comparative Endocrinology, Rutgers University, May 22-24, 1980.

Nicotra, Y., Needle, M., Ward, W., Bennun, A. and DeBari, V.  Transport of guanosine 3,,5' cyclic monophosphate cGMP into human erythrocytes. (1983). Biophysical Journal vol. 41, No. 2, Part 2, 163a.

DeBari, V.A., Bennun, A. and Needle, M.A.  Multiple internal reflectance-infrared spectroscopy (MIR-IR) of erythrocyte plasma membranes (Ghosts). (1983). Federation Proc. vol. 42, No, 7, 2552.

DeBari, V., Bennun, A.  Regulation of the pentose phosphate pathway in the human erythrocyte. (1984). Biophysical Journal, vol. 45, No. 2, Part 2, 212.

Cruz, R.A., Menendez, S. and Bennun, A.  The Role of energy charge in regulation of brain adenylate cyclase activity. (1984). Bull. New Jersey Acad. Sci. 29, 29-30.

DeBari, V.A., Needle, N.A. and Bennun, A.  Catalase as a manganese-dependent NADS4PH peroxidase.  (1984). Biochemical Society Bulletin vol. 6, No. 2, page 53 (609/32).

Bennun, A., Needle, N.A. and DeBari, V.A.  Infrared spectroscopy of erythrocyte plasma membranes. (1984). Biochemical Society Bulletin, vol. 6, No. 2 (609/33).

Sulner, J.W. and Bennun, A. Ca 2+-dependent control of renin release. (1984). Biochemical Society Bulletin, vol. 6, No. 3, page 30 (610/29).

Bennun, A., Seidler, N. and DeBari, V.A. A model for the regulation of haemoglobin affinity for oxygen. (1984). Biochemical Society Bulletin, vol. 6, No. 3, page 30 (610/30).

Sulner, J.W. and Bennun, a.  KCl-stimulated renin release. (1984). Abstracts Book - N.Y. Acad. Sci., Second Colloquium in Biological Sciences, p-39.

Bennun, a., Seidler, N. and DeBari, V.A.  Divalent metals in the regulation of hemoglobin affinity for oxygen. (1984). Abstracts Book - N.Y. Acad. Sci., Second Colloquium in Biological Sciences, p. 92.

DeBari, V.A., Cruz, R.A., Needle, N.A., and Bennun, A.  Dinucleotide oxidation by the Fenton reaction. March 5, 1985. Federation Proceedings vol. 44, No. 4, 922 (comm. 2937)

Sulner, J.W. and Bennun, A. Acachidonic acid stimulation of renin release:  Dependence upon calcium, calmodulin and B-adrenergic agonism (1985).  Federation Proceedings vol. 44, No. 4, 1127.

Bennun, A. Dependence Federation and Sulner, J.W.  Tyramine stimulation of renin release: upon calcium, calmodulin and prostaglandins. March 5, 1985. Proceedings vol. 44, No. 4, 1128.

Serban, G. Foldes, C. and Bennun, A.  Electrometric studies in human nails in vivo - 5th International Symposium on Bioengineering and the 5km (8/29-9/2, 19895).  Hyatt Regency Hotel, Union Square, San Francisco.

Cruz, Rafaela A., Billey, D., Bennun, A. and DeBari, V.A.  The Fe(II) - catalyzed peroxidation of nicotinamide adenine dinucleotides. (1986). The 14 th Annual Minority Biomedical Research Support Symposium, by Sponsored Div. Research Res. - National Institute of Health, New Orleans, Aprill6-20. NBRS Book of Abstracts, page B-32 comm. 191*

Cruz, R.A.*, Batista, V. and Bennun, A.  Integration of hormonal and endogenous regulation of adenylate cyclase.  The l4th Annual Minority Biomedical Research Support Symposium, sponsored by Div. research Res. National Institute of Health, New Orleans, April 16-22. 1986, NBRS Book of Abstracts, page 8-11, comm. 63.

Casciano, C. and Bennun, A.  Characterization of the interrelationship of the secretory activities of gastric tissues. Annals New York Acad. Sci., The Fourth Colloquium in Biological Sciences.  Vista International Hotel, World Trade Center, New York City, Nov. 3, 1986.  Book of Abstracts, comm. 53, 246-249.

Vicario, P. Saperstein, R., Katzen, H. and Bennun,, A.  Metabolic interactions of insulin-activated receptor kinase. Annals New York Acad. Sci., The Fourth Colloquium in Biological Sciences.  Vista International Hotel, 3 World Trade Center, New York City, Nov. 3, 1986.  Book of Abstracts, comm. 62.

DeBari, V.A., Needie, N.A., Bennun, A.  Catalase as a manganese-dependent NADPH peroxidase.  Cambridge Scientific Biochemistry Abstracts: Part 3. Amino-Acids, Peptides and Proteins.  Vol. 15, No. 3, Page 59 (1383-615).

Vicario, P., Saperstein, R., and Bennun, A.  Divalent metal ion interaction and activation of rat liver insulin receptor Tyrosine Kinase. 1987 Fed. Proc. 46, 2075 comm. 877.

Casciano, C. and Bennun, A.  A comparison of two inhibitors of H+K+ ATPase in gastric tissue.  (1987). Fed. proc.  Vol. 46 No. 6, 2106, comm. 1054

Bennun, A.  Hemoglobin as a model for a coupling mechanism between regulatory and active sites.  (1987). Fed. proc. 46, No. 6 2047, comm. 712

Cruz, R., Batista, V.M., and Bennun, A.  the integration of hormones, Mg2+  and Ca 2+  on the regulation of Adenplate Cyclase. NIR Centennial MBRS - Marc Symposium.  Arlington, Virginia, Oct. 1-3, 87 Book of Abstracts, comm. 317, page 72.

Batista, V.M., Cruz, R. and Bennun, A.  Divalent metals and hormones in the control of Adenplate Cyclase activity.  NIR Centennial NBRs - marc Symposium.  Submitted.  Arlington, Virginia, Oct. 1-3, 87 Book of abstracts, comm. 317, page 71

Casciano, C. and Bennun, A. A Characterization of Two Inhibitors of H+K+ATPase in Gastric Tissue. 623rd meeting of the Biochemical Society at the University of Kent at Canterbury 7/21-23/87.  Biochemical Society Bulletin, 9, No. 2, 66, comm. 52.

Vicario, P.P., Saperstein, R. and Bennun, A.  (1987) Regulation of insulin receptor tyrosine kinase (IRTK) by activatory Me+2, MeATP sybstrate and free ATP-4  .  623rd meeting of the Biochemical Society at the University of Kent at Canterbury.  7/20-23/87, Biochemical Society Bulletin 9, No. 2, 76, comm. 83.

Vicario, P.P., Saperstein, R. and Bennun, A.  (1987) Interrelationships of peptide substrate and metal-ATP with insulin receptor tyrosine kinase. 624th Meeting of the Biochemical Society at Dublin. Biochemical Society Bulletin 2, No. 3, 77, comm. 116

Vicario,P.P., Saperstein, R. and Bennun, A.  (1987) Kinetic studies of the reaction catalyzed by rat liver insulin receptor tyrosine kinase.  38th Annual Meeting of the American Physiological Society San Diego, Calif. 11/11-15/87, The Physiologist.  Vol. 30, No. 4, 128, comm. 8.4

Casciano, C. and Bennun A.  The Effect of Li + on the Secretion of HCO3  in Rat Fundic Tissue.  (1987). Biochemical Society Bulletin. Vol. 9, No. 3, 73, comm. 103.

Vicario, P.P., Saperstein, R. and Bennun, A. Metabolic feedback control of insulin receptor tyrosine Kinase. (1988). The FASEB Journal.  Vol. 2, No. 6, A1758 comm. 8490.

Casciano, C., Kaminska, G.Z. and Bennun A. Protection of rat gastric mucosa by two inhibitors of H K -ATPase against acid ethanol induced gastric lesions. (1988). The FASEB Journal.  Vol. 2, No. 4, A617 comm. 1863.

Casciano, C., Kaminska, G.Z. and Bennun, A. (1988).  Protection of rat gastric mucosa nu omhonotprs of  H+K+ against acid-ethanol induced gastric lesions IUB-l4th International Congress of Biochemistry, Praque, Czechoslovakia, July 10-15, 1988, Abstract book, accepted.

Vicario, P.P., R. Saperstein and A. Bennun. The interaction of rat liver insulin receptor tyrosine kinase with heavy metals. (1988). 626th Meeting of the Biochemical Society Bulletin 10, No. 1, 150, comm. 225.

 


III. PARTICIPATION IN SCIENTIFIC MEETINGS

8/7 – 8/12/66 - Interaction between chloroplast particles and coupling factor.  Latin American Association of physiological Sciences Mar del Plata, Argentina

11/21 – 1/12/61 - The effect of 2,4-dinitrophenol on the oxidation of endogenous substrates by the yeast Saccharomyces cerevisiae.  International Atomic Energy Agency. Conference "Use of Radio Isotopes in Animal Biology and the Medical Sciences

3/9 3/13/64 - Effect of DNP on the metabolism of phosphates in Saccharomyces cerevisiae.  Symp. of the Inter-American Nuclear Commission, Valparaiso

1967 – Interaction between chloroplast particles and coupling. Federation American Societies Experimental Biology, FASEB

6/1970 – Interaction of the chloroplast coupling factor with protons and water.  V Argentine Congress of Biological Sciences

6/24 – 6/29/71 - Sigmoidal kinetics of chloroplast's heat-activated ATPase.  Am. Society of Plant Physiology  Ind. Int. Cong. on Photosynthesis Research

1971 - Regulation of heat-activated Ca2+-dependent ATPase. Biophysical Society

9/14 – 9/17/71 - Properties of chloroplast's coupling factor-1.  European Biophysical Society (First Congress)

9/1972 - Bioenergetics of chloroplast.  N.A.T.O. Advanced study institute on "Primary Molecular Events in Photobiology" Fiesole, Italy, upon invitation

2/7 – 2/9/73 - The unitary hypothesis for coupling of energy transduction.  The New York Academy of Sciences invited lecturer to the Conference on the Mechanism of Energy Transduction in Biological Systems

7/1973 - Hypothesis for a biochemical memory pathway. International Union of Biochemistry, 9thInternational Congress

1974 - The unitary hypothesis and the relationship of enzyme modification to the pathophysiology of membranes. American Society of Biological Chemist, Inc. (Biochemistry/Biophysics)

 

9/2 – 9/6/74 - A model mechanism for coupled phosphorylation.  American society of Plant Physiology (Third International Congress of Photosynthesis)

1974 - Integration of hormone-dependent effects on adenyl cyclase.  American Chemical Society (168th) annual meeting

12/6/74 - “A unitary hypothesis of energy transduction and model mechanism for hemoglobin, coupled phosphorylation and active transport.  Berliner Physiologischen Gessellschaft and Akademie de Wissenschaffen der DDR (Invited lecturer)

12/9 – 12/12/74 - Properties of fat cell's adenylate cyclase.  Biochemische Gessellschaft der DDR, Dresden Congress in biological regulation (Invited lecturer)

1975 - Norepinephrine ad the pathophysiology of neuronal membrane.  Biophysical Society annual meeting

3/12/75 - "The role of energy activation in the regulation of the enzymes within the transducing membranes. International Union of Biochemistry (Plenary lecturer at the 7íst Symposium)

1975 - A model pathway for coupled phosphorylation. American Society of Plant Physiology.

1975 - Post-synthetic stabilized modification of adenylate cyclase by metabolites. The Biochemical Society (557th Meeting)

1975 - A metal coordinative chemistry model for coupled phosphorylation. American Society for Photobiology (annual meeting)

 

1975 - Relevance of the alteration of thresholds of adenyl cyclase response. American Chemical Society (l7Oth meeting)

11/1975 - Hormonal control adenylate cyclase in lipolysis. International Union of Biochemistry (plenary lecturer at 75th Symposium on the Regulation of Metabolic Pathways Bogota, Columbia)

1976 - Regulation of thresholds of adenylate cyclase response. Biophysical Society

1977 - A structure and function model for coupling between regulatory and active site. Biophysical Society

1978 - Metal and ATP interactions with adenylate cyclase. National Meeting Biophysical Society, Washington, D.C.

1980 - The effect of hormones on fat cell adenylate cyclase. Joint presentation with R. Cruz at Comparative Endocrinology Conference of American Society of Zoologist, Cook College, New Brunswick, N.J.

1980 - Regulation of the responsiveness to calcium of hypothalamic adenylate cyclase (with H. Ohanian). ASBC/BS fleeting, New Orleans

5/22 – 24/84 - The effect of hormones on metal and metal-ATP interactions with fat cell adenylate cyclase. Corresponding co-presenter R. Cruz. Eastern Regional Conference on Comparative Endocrinology Rutgers University

2/13 – 16/83 - Transport of guanosine 3', 5' cyclic monophosphate into human erythrocytes.  Corresponding co-presenter V. DeBari.  27th Annual Meeting Biophysical Society San Diego, California

 

6/5 – 9/83 - Multiple internal reflectance-infrared spectroscopy (MIR-IR) of erythrocytes plasma membranes.  Corresponding co-presenter V. DeBari. 74th Annual Meeting American Soc. of Biological Chemists, San Francisco, California

2/19 – 23/84 - Regulation of the pentose phosphate pathway in the human erythrocyte Corresponding co-presenter V. DeBari. 28th Annual Meeting Biophysical Society San Antonio, Texas

3/31/84 - The role of energy change on the regulation of brain adenylate cyclase activity Corresponding co-presenter S. Menendez

11/11/83 - Erythrocyte transport of cyclic nucleotides. Corresponding co-presenter V. DeBari.  N.Y. Acad. Sci. 1st colloquium in Biological Sciences.

6/84 - Catalase as a Mn2+ dependent NADPH erpxodase (609/32). Co-presenter V. DeBari.  609th Meeting of Biochemical Society, Leeds, England

6/84 - Infrared spectroscopy of erythrocyte (609/33). Co-presenter, V. DeBari. Biochemical Society, Leeds, England plasma membrane 609th Meeting

8/84 - Ca2+ - dependent control of renin release (610/29). 610 th Meeting Biochemical Society, Stirling, England

8/84 - A model for the regulation of haemoglobin affinity for oxygen (610/30). Co-presenter N. Scidler.  610th Meeting Biochemical Society, Stirling, England

11/5/84 - KCB-stimulated renin release (F-39). Co-presenter, J. Sulner. second Colloquium in Biological Sciences, N.Y. Acad. Sci.

 

11/5/84 - Divalent metals in the regulation of hemoglobin affinity for O2 (P-92).  Co-presenter, V. DeBari. Second colloquium in Biological Sciences, N.Y. Acad. Sci.

4/21 – 26/85 - Dinucleotide oxidation by Fenton reaction (Comm. 2987). Co-presenter, V. DeBari. 69th Annual Meeting FASEB, Anaheim, California

4/2126/85 - Arachidonic acid stimulation of renin release (Comm.4191). Co-presenter, J.W. Sulner. 69th Annual Meeting FASEB, Anaheim, California

4/21 – 26/85 - Tyramine stimulation of renin release (Comm. 4192).  69th Annual Meeting F'ASEB, Anaheim, California

8/31 – 9/86 - Session on haemoglobin, catalytic functions.  Sept. 2 10:15 to 10:45 a.m.  Title lecture:  A coupling mechanism to inter-relate regulatory with haem-haem interactions of haemoglobin.  XIth International Symposium on Structure and Functioning Erythroid Cells

 

 

IV. PROFESSIONAL ACTIVITIES

A. INVITED   LECTURES

1959 - Institute of Cellular Biology, School of Medicine, Univ. of Cordoba, Argentina

1961 - Seminar Program, Laboratory for Ceil Metabolism, National Atomic Energy Commission and Institute of Biochemistry, School of Medicine, University of Buenos Aires.

1961 - Department of Biochemistry, School of Pharmacy and Biochemistry, University of Buenos Aires.

1962 - Institute of Biochemistry, School of Medicine, University of Buenos Aires.

1964 - Seminar Program, Section of Biochemistry, Weizmam Institute of Sciences and Dept. of Molecular Biology, Neizmann Ist. of Sciences.

1965 - Dept. of Physiology and Pharmacology, Duke university, Durham, North Carolina

1965 - Biochemistry Section, Public Health Research Institute of the City of New York, Inc.

1967 - Seminar Program, Dept. of Biochemistry and Molecular Biology, Corneil University, Ithaca, N.Y.

1967 - Section of Genetics, Development and Physiology, Corneil University, Ithaca, N.Y.

02/67 - Charles F. Kettering Research Laboratory, Yellow Springs, Ohio (Lecture award)

1968 - Depart. of Biology, University of Puerto Rico Agricultural Station, University of Puerto Rico

1968 - Dept. of Biochemistry, Puerto Rico Nuclear Center, U.S.A. Atomic energy Commission (Lecture award)

1968 - Dept. of Chemistry, University of Puerto Rico, Rio Piedras

1969 - Seminar Program, Dept. of Zoology, NCAS, Rutgers University

7/70 - Section of Biochemistry, Institute of Microbiology “Malbran” Buenos Aires

7/70 - Dept. of Biological Chemistry, School of Medicine, University of Buenos Aires

7/70 - Institute Campomar, Buenos Aires

1/71 - Section of Biochemistry, Agricultural & Experimental Station, Rutgers University, New Brunswick

7/71 - Dept. of Biochemistry, Hebrew University, Jerusalem, Israel

6/71 - Dept. of Biochemistry, New Jersey College of Medicine arid nentistry, Newark

9/71 - Seminar Program Dept. of Chemistry, NCAS, Rutgers University

5/72 - Dept. of Biology, Newark State College

3/72 - The Institute for Enzyme Research, University of Wisconsin (Lecture award)

7/72 - Dept. of Biochemistry, School of Pharmacy and Biochemistry, university of Buenos Aires

1973 - Dept. of Biochemistry, School of Medicine, University of Puerto Rico (Lecture award)

7/73 - lecture at the Zentralinstitut fur Molekularbiologie, Akademie der Wissenschaften der DDR, Berlin Buch 1115, DDR

1974 - Zentralinstitut fur Molekularbiologie, Berlin Buch 1115, DDR

7/74 - Dept. of Biochemistry, School of Medicine, University of Buenos Aires

7/74 - Professional course and workshop in Metabolic Regulation. Biochemical Association, San Juan, Argentina, upon invitation to organize and teach the course

7/74 - Conference on television Estorneil Channel, Argentina

3/75 - Lecture award at the Chemistry Institute, University of Sao Paulo, Brasil

7/77 - Institute of Enzymology, School of Medicine, Universidad Autonoma, Madrid, Spain

12/77 - Biochemistry Department, School of Pharmacy and Biochemistry, University of Buenos Aires

12/77 - Lecture award at the Biochemical Association, San Juan, Argentina

12/78 - Department of Biological chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires

12/78 - Department of Biochemistry, School of Medicine, University of Buenos Aires

12/78 - Institute Campomar of Medical Research as guest of Director Luis F. Leloir, Nobel Laureate

1/79 - Lecture presented at Dept. of Biological Chemistry, School of Medicine, and Dept. of Biochemistry, School of Pharmacy, University of Buenos Aires

7/80 - Differential properties of adenylate cyclase from hypothalamus, corpus striatum and cortex. A. Bennun. Seminar, Dept. of Physio. and Biophys., C.I.E.A., natl. Polytech. Inst., Mexico City.

7/80 - Properties of fat cell adenylate cyclase".  A. Bennun. Seminar, Dept. Physiol. and Biophys., C.I.E.A., Natl. Polytech. Inst., Mexico City.

2/82 - Workshop in adenylate cyclase.  School of Pharmacy and Biochemistry, University of Buenos Aires

2/82 - Role of ionic equilibria in the control of adenylate cyclase activity. Department of Biological Chemistry, School of Chemical Sciences, University of Cordoba

3/82 - Dynamic controls of metabolic pathways. Biochemical Society of San Juan

5/82 - Workshop in interactions of coupling factors from chloroplasts with components of the chloroplast membrane. Biochemistry Section, Weizmann Institute of Science, Rehovot

12/83 - Adenylate cyclase - control mechanisms, workshop; School of Pharmacy and Biochemistry, University of Buenos Aires

1/84 - Hexose cycle in the erythroclyte, workshop, School of Pharmacy and Biochemistry University of Buenos Aires

1/85 - “Thermodynamics of hydrogen bondino and protein structure in hemoglobin and deoxyhemoglobin", workshop in the Division of Physical Chemistry, Department of Biological Chemistry, School of pharmacy and Biochemistry; University of Buenos Aires

9/85 - “A model for the regulation of hemoglobin affinity for oxygen"

9/86 - Department of Biochemistry and molecular Genetics, Pasteur Institute, Paris

9/85 - Divalent metals in the regulation of hemoglobin affinity for oxygen:, Department of Structural Immunology, Pasteur Institute, Paris

9/85 - "Hemoglobin structure and function", Biology Section, Curie Institute, Orsay, France

12/85 - Mechanism of cooperativity and hemoglobin function", Division of Physical Chemistry, School of Pharmacy and Biochemistry, University of Buenos Aires, Bs As.

12/85 - “Thermodynamics of hemoglobin function", research Department National Institute Mario Fatala Chaben, Ministry of Health and Social Action of Argentina, Bs As.

 

B. MEMBERSHIPS TO THE EDITORIAL BOARDS OF SCIENTIFIC JOURNALS

1975 – 1978 - Member of the Editorial Board of “Science of Biology Journal"

1978 – 1983 - Member of the Editorial Board of Journal of Bioenergetics and Biomembranes - Plenum Press

1976 - Reviewer for Biosystems

1979 - Reviewer for the Journal Cancer Biochemistry Biophysics

1983 - Present Reviewer for the Journal of Toxicology and Environmental Health

1983 - Present Member of the Editorial Board of Journal of Toxicology and Environmental Health, Hemisphere  Publishing Corporation

 

C. MEMBER OF THE FOLLOWING SCIENTIFIC SOCIETIES

 

1969 – 1982 - American Chemical Society, Division of Biological Chemistry

1969 – Present - American Society of Plant Physiology

1969 – 1981 - Genetics Society of America

1969 – 1974 - American Nuclear Society, radioisotopes and education divisions

1970 to Present - Biophysical society, Bioenergetics group

1970 to present - New York Academy of Sciences

1972 – 1974 - Federation of American Scientists

1972 – 1981 - Argentine Society of Biochemical Research (SAIR) and Pan American Association of Biochemical Societies (PAABS)

1974 – 1978 - American Society for Photobiology

1974 to present - The Biochemical society arid Federation European Biochemical Societies (FEBS)

1976 – 1978 - Society for Supramolecular Biology

1977 to present - American Society of Biological Chemists, Inc. and Federation of American Societies for Experimental Biology (FASEB)

1979 to present - American society of Zoologists

 

D. CHAIRING OF SCIENTIFIC CONFERENCES

Chairman, Biochemistry Platform Session 1, morning Annual Minority Biomedical Research Support (NBRS) the Division of Research Resotirces of the National The Fairmont Hotel, New Orleans, La., April 16-20, of April 17, the l4th Symposium, sponsored by of Health, Institutes 1986.

Chairman, Biochemistry Platform Session 2, afternoon of April 17, the l4th,Amual Minority Biomedical Research Support (KBRS) symposium sponsored by the Division of Research Resources of the National Institutes of Health The Fairmont Hotel, New Orleans, La., April 16-20, 1986.

 

E. ATTENDANCE TO ADVANCE SCIENTIFIC STUDIES INSTITUTES

1958 - U.S. department of State, Northwestern University and University of Buenos Aires, Advanced Study Program for the use of radioisotopes in Biology by Profs. J.A.B. Cooper and M. Lipton of the Northwestern Univ. in the Dept. of Biological Physics, School of Medicine, Univ. of Buenos Aires

9/72 -  N.A.T.O. Advanced Study Institute on "Primary Molecular Events in Photobiology", Fiesole, Italy, upon invitation

 

F. PROFESSIONAL. SERVICE

1970 - Has acted as reviewer for grant applications by request of the National Science Foundation

1973 - Served as National Institute of Health supported consultant for the School of Medicine, University of Puerto Rico

1973 - Member of the consultant organization of the Biophysical Society

1975 – 1976 - Member of the committee on Bioenergetics of the International Union of Pure and Applied Biophysics (IUPAB)

1981 - Consultant New Jersey Schools Board Association

1982 - Evaluator, Committee on an assessment of quality-related characteristics of Research-Doctorate programs, National Research Council, In progress.

1977 - Member, Public Health Panel.  New Jersey Association of Hispanic Human Services Professionals.  In progress

1980 - Chairman, Public Relations Committee.  New Jersey chapter of National Image, Inc.

1980 - Consultant NIH - In progress

1983 - Participant in the NIGNS, NIH funded “Visiting Scientists for Minority Institutions Program”. Listed in roster of available scientists.

 

G. MEMBER OF NON-ACADENIC INSTITUTIONS

1970 – 1972 - Sigma XI, Rutgers Chapter

1975 - Smithsonian Institution

1977 – 1979 - New Jersey Association of Hispanic Human Services Professions (NJAHHSP)

1978 – 1979 - International Platform Association

1978 – National IMAGE, Inc.

 

H. LISTED IN BIOGRAPHICAL PUBLICATIONS

American Men of Science, (1970) Bowker Co., 11th edition, supplement 6, p. 48, ACS Directory of Graduate Research, (1975)

 

I. BY NOMINATION

International Scholars Directory, International Scholarly publishers, first edition p. 17, 1972

Who's Who in the East, Narquis Who's Who, Inc., 1Sth edition, p. 50, 1975, 1976

1978 -  listing in special section of Who's who in America Comunity leader:and Noteworthy American:, American Biographical Institute, Bicentennial edition, pp. 69 & 70 1975, 1976

Dictionary of International Biography, International Biographical Center, Cambridge, England, p. 63, 1977

 


V. GRANTS RECEIVED

11/65 – 11/66 - USPHS Natl. Cancer Inst. (CA-87-66 supply allowance) Resolution and Reconstitution of chloroplasts:  $500

10/67 – 05/68 - University of Puerto Rico Research Fund Resolution and reconstitution  of chloroplast: $10,000 Technical assistance allowance $71600

09/68 – 08/71 - USPHS Natl. Inst. Genl. Medical Sciences 1-Rol GM 16390-91 Resolution and reconstitution of developing membranes: $18,860; 02: $10,798; 03: $15,885

09/69 – 08/79 - USPHS Natl. Inst. Genl. Medical Sciences Resolution and reconstitution of developing membranes: $15, 110

7/77 – Warner-Lambert Co. - Regulatory effects of hormones on adenylate cyclase from rat's fat cells; Research materials $16,000

10/77 – Mallinckrodt Nuclear - Research materials $2,500

8/69 – 8/77 - Research Corporation - Characterization of the functional properties of a structural resolved factor by the effect of its antiserum on photophosphorylation $8,000

11/75 – International Union of Biochemistry - Travel grant to 7Sth International Union of Biochemistry Symposium $200

1984 – 1986 - Bristo-Myer:  Estimated value - equipment $3,000; supplies $1,000

1985 – 1986 - Schering Plough Company - Estimated value - supplies $800

1986 - Hoffmann-LaRoche:  Estimated value equipment $18,200; supplies $1000

1984 - Develop contact, preliminary grant application, acted as Program coordinator for the initial NIH visitation and coordinator for the research units of Rutgers – Newark requesting participation in the Minority Biomedical Research Support Program (MBRSP) of NIH Division of Research Resources. The MBRSP application initiated by Dr. Bennun was awarded 9/27/84 as 6RS rant No. 1S06RR08223 01 to 00003 from 9/30/84 trough 9/29/87 to Hathannson, Dean of the Graduate School as principal investigator to administer the funding of about 12 associate investigator’s independent sub-projects working at several departments in NCAS. Direct costs: 1st year $107,370; 2nd year $223,792, and 3rd year $228,761 plus indirect costs

07/84 – 06/85 - Independent Associate Investigator College research award   $10,934 MBRSP (5S06RR08228)

06/85 – 06/86 - Independent Associate Investigator College research award   $13,241 MBRSP (5S06RR08228)

06/86 – 06/87 - Independent Associate Investigator MBRSP (5S06RR08228) College research award   $13,394 (Principal Investigator:  Dean of Graduate School-Newark whose 0ff ice administers the grant for the independent research associate investigator)

Minority High School Student Research Apprentice Program of the National Institutes of Health Biomedical Research Support Program at Rutgers, Newark *2S03RR03095-05:

1982 Allocation: Student $1,500; supplies - $500

1983 Allocation: Student $1,500; supplies - $500

1984 Allocation: Student $1,500; supplies - $500

1985 Allocation: Student $1,500; supplies - $500

1986 Allocation: Student $1,500; supplies - $300

 

 

Biomedical Sciences Support Grant:  USPHS FR7059:

07/69 – 05/70 - Resolution and reconstitution of biomembranes $1,0OO

06/70 – 05/71 - Resolution and reconstitution of biomembranes $400

06/71 – 05/72 - Resolution and reconstitution of biomembranes $1,000

06/72 – 05/73 - Interaction between coupling factor 1 arid ligands  arid their relevance to membrane phenomena $1,000

06/7305/74 - Hormonal-dependent modification of adenylate cyclase $800

06/75 – 05/76 - Hormonal - dependent modification of adenylate cyclase $600

04/76 – 03/77 - Hormonal and metabolic control of adenylate cyclase in rat's brain $600

04/77 – 03/78 - Hormonal regulation of adenylate cyclase $600

04/78 – 03/79 - Hormonal regulation of adenylate cyclase $600

04/79 – 03/80 - Hormonal regulation of adenylate cyclase $800

04/80 – 03/81 - Hormonal regulation of adenylate cyclase $800

04/81 – 03/82 - Hormonal regulation of adenylate cyclase $800

04/82 – 03/87 - Regulation of renin release $500 every year

 

Rutgers Research Council Grants

06/89 – 06/70 - Resolution and reconstitution of biomembranes $2,500

07/70 – 06/71 - Travel grant Photobiology Symposium of the V° of Biological Sciences $500

08/72 – 05/73 - Travel grant $500

12/72 – 05/73 - Physio-chemical forces that result in modification of enzyme activity $1,000

06/73 – 05/74 - Interaction between coupling factor and ligands $750

06/77 – 05/78 - Hormonal control of rat's fat cells adenylate cyclase $500

06/81 – 05/82 - The Effect of hormones on adipocyte adenylate cyclase $500

 

Charles and Johanna Busch Memorial Fund

1974 – 1975 - The role of enzyme modification in the friction and panthophysiology of membranes $6, 500

09/74 – 11/74 Visiting scientist under the Academic Leave Program of Rutgers University at the Laboratoire d'Enzimologie Centre National de la Recherche Scientifique France

 

FASP Grants Committee

07/78 – 12/78   Hormonal regulation adenylate cyclase system $500

 

Grants to Obtain Grants

04/78 – For general support $260

 


SCHOLARLY/CREATIVE ACTIVITIES

 

VI. TEACHING

1957 - Research technology, non-credit course, Dept. of histology and Embryology, School of Medicine, Univ. of Cordoba, Argentina

1958 - Sections of Experimental Biochemistry, Dept. of Biochemistry, School of Biochemistry, University of Buenos Aires

1958 – 1962 - Sections of Biological Chemistry, School of Medicine, University of Buenos Aires

1967 - 1969 - Biology 103-104, General Biology, Dept. of Biology, University of Puerto Rico

1967 - 1969 - Biology 379, Reading and Research, Dept. of Biology, University of Puerto Rico

1968 - 1969 - Biology 354, Cellular Biochemistry, Dept. of Biology University of Puerto Rico

1968 - 1969 - Biology 257, Plant Physiology, Dept. of Biology, University of Puerto Rico

 

990:494 Seminar in Zoology (1 cr)
Fall 1970, Spring 1976, Spring 1977.

115:413 Experimental Biochemistry (2 cr)
Fall 1971, Fall 1972, Fall 1973, Fall 1975, Fall 1980.

990:411 Endocrinology
Fall 1977.

115:414 Experimental Biochemistry (2cr)
Spring 1972, Spring 1973, Spring 1974.

990:204 Physiological Zoology (4 cr)
Spring 1976, Spring 1977, Spring 1978

120:101 General Biology Laboratory
Fall 1973, Spring 1974, Fall 1986

115:310 Elementary Biochemistry
Spring 1980, Spring 1984, Spring 1985, Spring 1986.

115:312 Elementary Biochemistry
Fall 1980, Fall 1983, Fall 1984, Fall 1986.

115:404 General Biochemistry
Spring 1980, Spring 1981, Spring 183, Spring 1984, Fall 1984, Spring 1985.

760:407 General Physiology
Fall 1981, Fall 1982, Spring 1979, Spring 1983.

115:405 Problems in Biochemistry
Fall 1982, Fall 1983, Spring 1984, Spring 1985.

990:491 Special Problems in Zoology
Fall 1982, Spring 1983.

 

Courses Taught (at Graduate School Rutgers University)

115:513 Metabolic Pathways Controls (3 cr)
Fall 1969, Fall 1970, Fall 1971, Fall 1972, Fall 1973, Fall 1975.

990:513 Metabolic Pathways Controls (3 cr)
Fall 1975, Fall 1976, Fall 1977, Fall 1979.

115:514 Metabolic Pathways Mechanisms (3 cr)
Spring 1970, Spring 1971, Spring 1972

990:514 Metabolic Pathways Mechanisms (3 cr)
Spring 1973, Spring 1974, Spring 1976, Spring 1977, Spring 1978.

990:511 Advanced Endocrinology (3 cr)
Fall 1976, Fall 1977, Fall 1979, Fall 1980, Fall 1981.

990:616 Special Topics in Biochemistry (1 cr)
Fall 1975, Spring 1976, Spring 1977, Fall 1977, Spring 1978, Spring 1979, Spring 1984, Fall 1984, Spring 1985, Fall 1985.

115:606 Advanced Studies in Biochemistry
Spring 1974

115:701 Research in Zoology
1969 to Present

990:509 Advanced Problems in Zoology
Summer 1976, Spring 1977, Summer 1978, Spring 1979, Fall 1978, Fall 1985.

160:572 Biochemistry
Spring 1980, Spring 1981, Spring 1984, Spring 1985, Spring 1986

 


Supervision of Honors Thesis Completed by Students in the Undergraduate Honors Program:

1969 1968 -        W. Velez-Vega and M. Dieppa - Honors Program Advisor -Biology Department University of Puerto Rico

1981 - Nancee A. Novak - The effect of cyclic nucleotides upon glycolysis in the human erythrocyte

1982 - Bessie B. Fouces - Kinetic studies on brain adenylate cyclase

1984 - Sylvia Menendez - The role of energy charge in the regulation of brain adenylate cyclase activity

 

Summer Supervision of Minority High School Students with Research Apprentice Program of NIH-BRSP

1982  Patricia Grady

1983  Patricia Grady

1984  James Anderson

1986  Manuel Dasilva

 

VII. UNIVERSITY SERVICE

1967 – 1969 - Member of the Graduate Faculty, Department of Biology, University of Puerto Rico

1970 - Full member of the graduate School of Rutgers University.

1970 - Full member of the cooperative Graduate Program in Biochemistry, Rutgers University.

1970 – 1973 - Member of the University Science Council, Rutgers University

1975 - . Member of the Academic Standards Committee of the Cooperative Graduate Program of Biochemistry.

1970 - Member of several graduate students’ committees in the Graduate Program in Biochemistry

1970 - Full member Graduate Program in  Zoology. NCAS

1970 - Member of several graduate students  committees in the Graduate Program in Zoology NCAS

1975 – 1978 - Member of the NCAS Academic Affairs Committee

1977 – 1978 - Member of NCAS Graduate School Planning Committee

1979 – 1981 - Member of NCAS Graduate School Planning Committee

1977 – 1978 - Member of Curriculum Committee, Dept. of Zoology and Physiology NCAS

1978 - Member of Executive Committee of the Graduate Program in Zoology NCAS

1979 - Member of Teaching Evaluation Committee NCAS

1978 - Member , Cancer Coordinating Committee of Rutgers, Newark NCAS

1983, 1985 - Member, Executive Committee Graduate Program in Zoology NCAS

1984 – 1986 - Member of the advisory committee for MBRS program in Newark Campus of Rutgers.

 

 

VIII. GRADUATE STUDENT SUPERVISION

Supervision of Ph.D. candidates research and titles of Ph.D. dissertations completed by students under my direction and initial post-doctoral positions:

 

Susan B. Golz - Regulation of brain adenylate cyclase by physiological and pharmacological factors.  Ph.D. in Zoology awarded May 1976. Initial Position: Assistant Professor, Bergen Community college.

Robert H. Harris - Hormonal regulation of rat fat cell CAMP levels and fat ceil membrane adenylate cyclase.  Ph.D. in Biochemistry awarded November 1977.  Initial Position: Research specialist, Division of Metabolism of Food and Drug Administration, Bethesda, MD.

Heripsime Ohanian - The role of divalent metals, adenosine 5'-triphosphate and hormones in the regulation of rat brain adenylate cyclase.  Ph.D. in Zoology awarded June 1980.  Initial Position: Medical School, Lille University, France.

Vincent A. DeBari - Regulation of the hexose monophosphate pathway in cyclic nucleotides and divalent cations in the human erythrocyte. Ph.D. in Zoology awarded May 1981. Initial Position: Director, Renal laboratory, St. Joseph's Hospital, Paterson, N.J.

Joseph W. Sulner - Renal renin release - a stimulatory role for Calcium Ph.D. in Zoology awarded May 1984.  Postdoctoral Hoffman – Laroche. Initial Position: Associate Investigator N.Y. Veteran's Hospital.

Rafaela Cruz - The integration of hormones, Ng 2+ Ca2+ and their nucleotide complexes on the regulation of brain adenylate cyclase. Ph.D. in Zoology awarded October 1986. Initial Position: Adjunct Faculty in Anatomy and Physiology at Upsala College, East Orange, N.J..

Pasquale Vicario – The regulation of Insulin Receptor Tyrosine Kinase by Insulin, Divalent metal cations and metal – ATP substrate. Ph. D. in Zoology awarded May 1988. Initial Position: Investigator at Merck Company.

Christopher Casciano – The pharmacological characterization of the bicarbonate, proton, and mucus transport systems of the rodent gastric mucosa and their significance in cytoprotection. Ph. D. in Zoology awarded September 1988. Initial Position: Investigator at Schering Company.

 

Supervision of Ph.D. candidates research under my direction:

1984  G. Serban

1985  P. Uychich

1985  L. Rumennick

Supervision of Masters candidates research and Masters Thesis completed by students:

1975 - Chairman of the committee for the Master in Zoology of Susan Brydon Golz. M. Sc. awarded 1975.

1978 - Chairman of the committee for the Master in Zoology of Heripsime Ohanian. M. Sc. awarded 1978.

1981 - Chairman of the committee for the Master in Zoology of Rafaela Cruz. M. Sc. awarded 1981

Supervision of research:

1968 – 1969 - Advisor in the Biology Program to 1. Bulla, M.S. candidate at university of Puerto Rico

1970 – 1971 - Advisor in the Graduate Program of Biochemistry to E. Albanese and V. Cannistraro.

1970 – 1971 - Advisor in the Master in Zoology Sciences Program to E. Wallendjack

1977 - Research advisor to Kamran Borhanian, student at NJCMD, Rutgers Medical School

1977 - Postdoctoral research advisor to Salette de Farias on a leave from Institute Butantan, Sao Paulo, Brazil

1984 - Research advisor to Norbert Seidler, student at NJCMD, Rutgers Medical School

Membership on Graduate Student Committees:

1974 - Vincent Rama (Ph.D., Zoology)

1977 - Jeff Stevens (M.S., Biochemistry)

1980 - Jeff Stevens (Ph.D., Biochemistry)

1979 - Rosy Jordan (M.S., Zoology)

1979 - Gerald Costa (M.S., Zoology)

1980 - Robert Jonathan Weiss (M.S., Zoology)

1980 - Beryl Joyce Carby (M.S., Zoology)

1980 - Vera Minak (M.S., Zoology)

1980 - Frank R. Settineri (M.S., Zoology)

1981 - Diane G. Verga (M.S., Zoology)

1983 - Dolores Curtis (M.S., Zoology)

1983 - Marlene Blanco (M.S., Zoology)

1983 - Mark Schachman (M.S., Zoology)

1983 - Thomas Brankner (M.S., Zoology)

1984 - Lee D. Carpe (Ph.D., Zoology)

1984 - Dennis Guiliani (Ph.D., Zoology)

1985 - Eva-Pia Reich (Ph.D. Zoology)

1985 - Ilda D. D'Onofrio (Ph.D. Zoology)

1988 - John C. Anthes (Ph. D. Zoology)

 

IX. RESEARCH ACCOMPLISHMENTS

A. Active Transport and metabolic control

a)       It was demonstrated that active transport of phosphate by yeast is directly dependent on high levels of energy – rich phosphates maintained by either endogenous oxidation or by exogenous substrates during oxidation by either glycolysis or respiration.

b)      It was shown that yeast’s uptale of acetate, pyruvate, etc. and their oxidation rate within the Krebs cycle, rather than under direct control by oxygen itself, is subject to an energy dependent regulatory mechanism supported by ATP levels and their equilibria with other energy rich phosphate compounds.

 


B. Mechanism of oxidative phosporylation

         In 1963, the DNP – activated, Mg – dependent ATPase of yeast was for the first time isolated and characterized for its role in oxidative phosporylation.

III. Mechanisms of enzymatic induction and reversion as cells adaptative response to environmental changes.

         It was found that Wuglena controls endogenous phosphatase levels by a low phosphate contration – dependent induction of the synthesis of a phosphate. Induced and constitutive phosphataase were isolated and characterized as differente enzymes. It was found that disappearance of the induced enzyme (reversion) is a high phosphate shows a temperature – dependent function. The low activation energy (heat) required for the denaturation of the induced enzyme in solution allows to postulate that cell’s reversion operates through a high phosphate – dependent thermic sensitization of the induced enzyme which accelerates its thermic decay.

 

C. Mechanism of photophosphorylation

a)     In 1964, the proposition that high energy intermediates should be equated with conformational changes was published. It was proposed that conformational change  participated in energy conservation and transduction. Ca2+ and cysteine wiche eleicit light – requiring ATPases were shown to, modify the kinetic behavior of the ATP synthetase. ADP and GDP show inhibitory constants (Ki) for the light – requiring ATPases of the same numerical value that their Kms for residual and modified phtophosphorylation. This would suggest the participation of a single, but modigiable, active center in the mechanism which would determine the direction of the ATP synthetase activity

b)    The first successful resolution and reconstitution of an energy – dependent ATPase function was achieved for chloroplast light – requiring ATPases. Isolation and purification of the ATP-synthetase choroplast’s coupling factor. It was also shown that the latter becomes an ATPase by heat or trypsin treatmen.

c)     Reso1ution and reconstitution of the functlon for metal-dependent binding of the coupllng factor (ATP synthetase-ATPase) by the chloroplast membrane. Characterization of allotopic properties of the system.  Characterization of the Inhibitory capacity by the membrane on the ATPase activity during binding.

d)    The finding of the capability of the ATPase to maintain cooperative interaction with 2 ATP, 4PO4, 2 ADP and 16 molecules of water, was used to postulate that the active form of the ATPase is a structure characterized by conserving the energy of 14 H-bonds or 14 Ki1oca1ories (1 kilocaloráe per each solvating water molecule), where as that of the inhibited form of the ATPase is a estructure characterized by a decrease in energy content of the same value. This appears to indicate the potential of the ATPase itself to function as a high-energy intermediate during the turnover of the ATP synthetase system.

e)     First report on the allosteric kinetics of the ATPase (activated coupllng factor), and demonstration that proton concentration has the capability to requlate the modification of the ATPase from a form of high affinity for ATP and low affinity for phosphate and ADP, into another form of opposite properties.

 

D. Energy Transduction

 

a)     Kinetic and thermodynamic parameters were described for the operatien of ATPase coupled for ionic transport, muscle contraction, interaction between cytochromes and coupling factor (ATPase) and the effect of ions in the modification of chloroplast’s light-dependent activities.

b)    The postulation of energy transduction mechanisms resulting from the exergonic association of proteins with divalent metals (Me2+). The turnover of the protein te a dissociated state (endergonic) is driven by the chelation of the binding Me2+ by ATP4- (exergonic). ATPase activiy turns ATP4- into the waker chelating agent ADP3- allowing Me2+ dissociation and the return te the initial high concentratien of free Me2+.

c)     The binding of a divalent meta1 requirement for the EDTA-treated membranes binding of the coupling factor-ATpase led to the postulation that electron transfer is coupled to phosphorylation by cytochreine-dependent or pH-dependent modificatien of the meta1 chelated states of R-groups located in the coupling site of the ATPase.

d)    Consequently, the unitary hypothesis can be differentiated from the chemical hypothesis in that it postulates the formation of high energy intermediates by the transfer of groups across coordinative bonds rather than across covalent bonds. It also integrates conformational and chemiosmotic prepositions within a single framework.  Accordingly, the hypothesis uses a single thermodynamic and kinetic framework for findings that were previously explained only through the alternate use of three different hypothesis.

 

E. Hemoglobin

It has been previously proposed that in an open thermodynamic system, the turnover of the state of Me2+ as ligand of pretein(s) would yield the energy of activation for driving conformational and functional changes of protein.  Accordingly, it was proposed that oxygenation of Hb would lead Me2+ induce-fit a chelating configuration of side chains. This binding of Me2+ to Hb would dislocate proxima1 histidines from the positions reguired for steric hindrance and would allow Bohr side chanin(s) te release their proton(s).  Mg2+ rather than Zn2+, was suggested as the main physiological Me2+ to compete with Zn2+ at cellular concentrations of 11 ug/g red cells for the chelating side-chains of Hb. Oxygenation induces binding of Mg2+ or Zn2+   to Hb. Hence, according to the mode1 the PH-dependent stability order of the chelated complexes during deoxygenation would be: (Zn2+)2-oxyHb>(Zn2+, Mg2+)-oxyHb>(Mg2+)2-oxyHb

 

F. Characterization of the hormonal and metabolic parameters that control adenylate cyclase activation

 

a)     GH potentiates the effect of epinephrine, glucagon and theophylline increasing endogeneus cAMP levels in fat celis.

b)    Isolated membranes show that epinephrine activates adenylate cyclase by decreasing its Km for ATP and its Km for Mg2+ to reach a maximal activatory effect. Adenylate cyclase can be described as a receptor-coupled enzyme system in where the hormone binding by the receptor increases the affinity of the enzyme's regulatory site for Mg2+. The regulatory site also functions in the absence of epinephrine because high Mg2+ concentration is also capable of activating adenylate cyclase (19)

 

G. Characterization of hormonal and metabolic parameters that desensitize and inactivate adenylate cyclase

 

a)     It was found that an adenylate cyclase membrane preparation from rat's cerebral cortex, during prolongued preincunation at 37 C, became rapidly inactivated when noradrenaline was present.  Total loss of activity was reached at 100 minutes.  ATP was able to protect the enzyme against this effect for over 4 hours.  On the other hand, ATP alone activated the enzyme but desensitized it to its stimulation by noradrenaline.  The characterization of the described effect indicates a noradrenaline-dependent thermic sensitization which accelerates thermic decay. ATP protects against this effect.  Hence, It was surmised that since anxiety and stress are mediated by catecholamines, prolongued maintenance of high noradrenaline levels in conditions that exhaust endogeneus ATP in the affected organs, may inactivate adenylate cyclase and explain, therefore, the aetiology of psychosomatic and stress related diseases  (i.e. anxiety leading to depression).

b)    Solving multiple-equilibirum equations demonstrated that the relationship between energy charge (ATP vs. ADP and AMP) and relative concentration of free vs. chelated by nucleotides divalent metals (Me: Mg2+, Mn2+, Ca2+) plas a modulatory role for adenylate cyclase and insulin-dependent tyrosine kinase at three sites (substrate site, Me2+-dependet regulatory site and Me-requiring site for control of hormone responsiveness)

 

 

H. Metabolic controls

The cellular uptake of glucose and/or the lysis of glycogen it is expected to change the concentrations of chelating metabolites (sugar phosphates) and therefore the relative concentrations of free versus chelated divalent metals.  Thus a decrease in chelating metabolites constitutes an amplification mechanism of the adenylate cyclase (AC) response, since it simultaneously increases the concentrations of the substrate (MgATP) and the positive modulator (Mg +2) and decreases that of the inhibitor (ATP 4-).   Mg2+ or Mn2+, in contrast with Ca2+, may control not only the basal activity, but also the hormonal responsiveness of AC. A more comprehensive description of metabolic shifts requires the examination of hormonal action and metabolic feedback not only on AC, but also on insulin receptor tyrosine kinase (IRTK).  The metal dependence of IRTK, as well as the effect of changes on chelated species of ATP on this dependence, were investigated to elucidate the integrative parameters which allow coordinated hormonal, ionic and energy-charge control of the metabolic shift from glycolysis to gluconeogenesis.

 

I. Control mechanisms for secretory processes:

a) Renin release in kidney tissue.

b) HCl versus bicarbonate in gastric tissue

 


MAJOR RESEARCH INTERESTS

Metabolic Regulation: in yeast Energy transduction: in chloroplast and mitochondrial membranes, resolution and reconstitution, ATAase

Control mechanisms of enzyme-membrane systems: hormone receptors-adenylate cyclase systems in fat cells and brain membranes, insulin receptor tyrosinekinases in liver.

 

Modeling of Energy trans­duction mechanism: Hemoglobin

Areas of Specialization - Cellular actions of hormones Enzyme kinetics Cellular structure and function Membrane biochemistry of organelles, chloroplasts and mitochondria