Hormonal
control of fat cells adenylate cyclase
Harris, R. and Bennun, A
Fat cells were
preincubated for 2 h in the pres¬ence and absence of growth
hormone (GH) and Dexamethasone (Dex) before the addition of
increasing concentrations of either epinephrine. theophylline
or glucagon and final incubation of the cells for an additional
5 min¬utes. GH and Dex increased by 85%, 28% and 72%,
respectively, the cAMP levels reached in the sole presence
of 10-5 M epinephrine, 10-2 M theophylline or 5 x 10-5 M glucagon.
An adenylate cyclase particulate pre¬paration shows that
epinephrine decreases Km from 2 mM to 0.6 mM and increases
Vmax and the strength of interaction value (n) from 0.91 to
1.75.
Molecular & Cellular Biochemistry, 13, (1976), No. 3,
141-146